Fig. 8
From: Multi-omics cluster defines the subtypes of CRC with distinct prognosis and tumor microenvironment
Validation the function of MID2 in CRC cell lines. A The expression level of MID2 in different stages of CRC patients. B GSEA analysis demonstrates enrichment of MID2-related genes in the epithelial–mesenchymal transition (EMT) pathway. C SW480 and HCT116 cells were transfected with plasmid carrying sh-NC or sh-MID2. The protein level of MID2 and EMT biomarkers were assessed using western blotting. D Colony formation assay to evaluate the effect of MID2 knockdown on the proliferation of CRC cells. E–F Representative images and the comparison of cell migration and invasion ability demonstrated by transwell assay between sh-NC and sh-MID2 cells based on SW480 (E) and HCT116 (F) cell lines. The scale bar represents100 μm. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and two-tailed unpaired t-test)