Necrotizing fasciitis: microbiological characteristics and predictors of postoperative outcome
© I. Holzapfel Publishers 2009
Received: 17 June 2008
Accepted: 22 July 2008
Published: 28 January 2009
Necrotizing fasciitis is a life threatening soft-tissue infection with a high morbidity and mortality. Prompt treatment based on extensive surgical debridement and antibiotic therapies are the therapeutic principles.
The medical records of patients with necrotizing fasciitis (n = 26) from 1996 to 2005 were retrospectively analyzed.
The localization of necrotizing fasciitis was most commonly the trunk (42.3%). Type I polymicrobial infection was the dominating infection. The involvement of anaerobic bacteria was associated with an increase in the number of surgical revisions (p = 0.005). Length of postoperative intensive care unit stay, duration of postoperative ventilation and mortality were significantly increased in the ASA IV-V group. Computed tomography displayed only a limited significance as diagnostic tool for initial diagnosis.
In severe cases the combination of necrotic skin and soft tissue gas facilitates the correct diagnosis, which should than be followed by immediate - and most often - repeated debridement. If anaerobes are isolated an early and aggressive second look is necessary.
KeywordsNecrotizing fasciitis soft tissue infection ASA classification
Necrotizing fasciitis is a rare but aggressive soft-tissue infection, which involves the fascial layers and the subcutaneous tissue, while skin and muscle initially remain intact . This infection, which is usually induced by virulent, toxin producing bacteria, can occur in any region of the body but it is predominantly located in the abdominal wall, perineum and extremities [2, 3]. On the basis of microbiological cultures Giuliano and colleagues  divided the necrotizing fasciitis into two distinct groups. Type I infections are polymicrobial and involve non-group-A streptococci, aerobes and/or facultative anaerobic bacteria. Type II is usually caused by β-haemolytic group-A streptococci alone or in combination with staphylococci. Predisposing factors of the disease are diabetes, alcohol and intravenous drug abuse as well as immunosuppression and peripheral vascular disease [5, 6]. However, necrotizing fasciitis has also been reported in young and previously healthy individuals.
An early sign of necrotizing fasciitis is local erythema and pain out of proportion to findings of physical examination as well as fever. Skin necrosis due to thrombosis of blood vessels at the fascial level is a sequel of the initiating process and is often associated with severe sepsis [7–10]. Histomorphological hallmarks are necrosis of the superficial fascia with blood vessel thrombosis, severe inflammation of the dermis and subcutaneous fat as well as subcutaneous fat necrosis. Sometimes myonecrosis of underlying skeletal muscle is obvious [11–13]. Diagnostic clues include clinical findings and detection of soft-tissue gas or in less obvious cases edema and stranding of the fascial layers by computed tomography (CT) scanning as well as soft-tissue fluid by magnetic resonance imaging (MRI) [14–17].
Because of the high mortality ranging between 6 and 76% in different reports, prompt and aggressive therapy based on early and extensive surgical debridement, antibiotics and intensive care is necessary .
The aim of the present study was to analyze the factors affecting the outcome of patients with necrotizing fasciitis treated at our institution over the past 9 years. At the beginning of this time period a necrotizing fasciitis alertness "attitude" was instituted, presenting every dubious case to particularly involved staff surgeons.
Materials and methods
The medical results of patients who had been treated at our department for necrotizing fasciitis between 1996 and 2005 were analyzed retrospectively. The diagnosis of necrotizing fasciitis was established based on clinical findings such as characteristic skin changes, pain out of proportion to physical findings, fever, leucocytosis and elevated c-reactive protein levels as well as intraoperative findings such as necrosis of the superficial fascia and fat and the presence of foulsmelling fluid known as "dishwater pus".
In 10 cases (n = 10) a CT scan was performed. CT examinations were reviewed separately and blinded by two radiologists. To assess the predictive value of CT, CT scans from 10 patients with other soft tissue infections such as cellulitis and phlegmonia were included in the radiological review. All pathological changes were noted including those of the cutis, subcutis, fasiacl layers and muscle. In cases which remained unclear despite these examinations suspected foci were exposed surgically and the procedure was chosen according to the clinical aspect of the tissue. If the diagnosis was not adequately established by these methods, a biopsy specimen was analyzed by frozen section or rapid embedding process and measures were taken accordingly, e.g. a mild non mutilating debridement until verification of the diagnosis by histopathological examination. All cases included in this study finally had histopathological verification of the disease.
We assessed the value of the different diagnostic procedures performed in each case before radical debridement. We also reviewed the medical results for impact of demographic and clinical data as well as laboratory findings at the time of admission, comorbidities, site of infection, time from symptoms to admission, number and type of operations, the duration of hospitalization and the length of intensive care unit (ICU) stay as well as days on the respirator. The patients were classified by organisms isolated from swabs or tissue specimens during the first surgical treatment as type I and type II infections according to Giuliano and associates . The physical status of the patients before the first surgical treatment was assessed according to the criteria of the American Society of Anaesthesiologists (ASA) .
Data analysis was performed using the SPSS 11.5 software package (SPSS Inc., Chicago, IL, USA). Correlation between epidemiological, clinical and microbiological variables was examined using the nonparametric Mann-Whitney-test. A two-tailed p-value less than 0.05 was considered to indicate statistical significance.
Spectrum of comorbidities in patients (n = 26) with necrotizing fasciitis
No. of patients [%]
Chronic inflammatory bowel disease
Chronic renal insuficiency
Autoimmune hemolytic anemia
Peripheral vascular disease
Mixed tissue disease
Clinical and biochemical findings on admission in patients (n = 26) with necrotizing fasciitis
Clinical and Biochemical findings
No. of patients [%]
Microbiological isolates among patients (n = 26) with necrotizing fasciitis
Necrotizing fasciitis is a life threatening, rapidly progressing soft tissue infection with a high mortality up to 76%. However, early diagnosis and extensive surgical debridement of all necrotic tissue has been demonstrated to improve survival [7, 8, 19, 20]. Paucity of clinical findings in the early course of the infection makes a timely diagnosis difficult. Early signs of disease do not include skin necrosis, crepitus or dishwater pus, in particular in type II infections.
The therapy has to be initiated as early as possible, but radical skin debridement in patients who turn out not to suffer from necrotizing fasciitis has to be avoided. Therefore further diagnostic tools such as imaging procedures or histopathologic examination have to be used. Yen and colleagues  applied the criterion of diffuse thickening of the subcutaneous tissue accompanied by a fluid layer amounting more than four millimetres along the fascia in a prospective study using ultrasound techniques. They found a sensitivity of 88.2% and a specificity of 95.4%. More frequently used, CT scan may recognize fasciitis by detecting diffuse enhancement and thickening of the superficial and deep fascia as well as soft tissue gas or fluid collections [14, 15]. Consistent with previous reports typical diagnostic features at CT in our group were fascial thickening with enhancement and edema of the adjacent muscle, as well as fat stranding, fluid collections and gas tracking along fascial planes. CT scanning might help the surgeon to show the extension of an infectious process, but was in our group limited in its validity to distinguish between necrotizing fasciitis and other soft tissue infections. More suitable in distinguishing between necrotizing fasciitis and cellulites seems to be MR imaging with a reported sensitivity of 100% and a specificity of 86% performed in a group of seventeen patients consisting of eleven cases of necrotizing fasciitis and six cases of cellulites . Thus, supporting that MR imaging rather than CT scanning should be preferred as diagnostic tool in necrotizing fasciitis. To establish the diagnosis of necrotizing fasciitis the correlation of clinical, imaging, and laboratory findings is mandatory. As formerly described, a frozen-section or rapid embedding biopsy of suspicious lesions is useful for early definitive diagnosis and can therefore reduce mortality significantly [22, 23].
Our operative strategy is aggressive debridement with complete removal of all necrotic areas until healthy tissue is exposed. Skin flaps are moderately trimmed and separated from the underlying tissue by surgical towels. Necrotic areas are resected. A daily examination of the wound must be performed in order to asses the local conditions and to exclude a progression of infection and necrosis.
Most of our patients presented on admission with typical symptoms of early necrotizing fasciitis such as swelling, erythema, pain out of proportion to physical examination and fever. Signs of a more progressive stage of the disease such as skin necrosis, purulent secretion, hemorrhagic bullae formation, crepitus and signs of a compartment syndrome as well as sensory and motor deficiencies were observed only in a minority of patients. Histopathologically, necrosis of the superficial fascia combined with angiothrombosis and suppuration are characteristic [11, 12]. Thereby subcutaneous fat necrosis mostly without major changes of the epidermis occurs as well as inflammation of the dermis and subcutaneous fat with infiltrating leucocytes. Bakleh and associates  concluded from a group of 63 cases with necrotizing fasciitis that histopathological findings and clinical course may correlate and established a model of three stages.
In our study the most common infection was a type I-polymicrobial infection with a prevalence of 53.8%. In 30.8% of our patients with type I infections, cultures grew also anaerobes. Patients with positive anaerobic cultures needed more surgical revisions, suggesting that especially these patients might profit from a hyperbaric oxygen therapy (HBO) in the adjuvant therapy of necrotizing fasciitis. A reduction of mortality and a decreased need of debridements were reported [24–27]. In particular, patients suffering from infections with anaerobes may benefit from HBO therapy because of the direct toxic effect of increased free radicals through increased oxygenation in the hypoxic tissue area .
The classification of physical status according to the American Society of Anaesthesiologists  correlates with perioperative mortality or postoperative outcome [29, 30]. So far, the prognostic value for patients suffering from necrotizing fasciitis had not been shown. We found a correlation between ASA status and days on the ICU, days on the respirator, and survival.
Necrotizing fasciitis remains a major challenge for several reasons. While it is of utmost importance to diagnose the disease early in its course, the signs may be very unspecific and less severe conditions such as cellulitis or may be considered. Once the suspicion of necrotizing fasciitis is raised immediate action is required. The diagnosis and extent have to be established. Resection of all affected fascia may require the resection of large segments of viable skin and subcutaneous tissue. The problems are particularly striking in type II necrotizing fasciitis, where typical foul-smelling dishwater pus is missing.
In order to overcome these difficulties, we instituted a "necrotizing fasciitis alertness" team, including an experienced staff member, which is supposed to see all patients who are under suspicion immediately after admission. If immediate diagnosis cannot be established, diagnostic procedures like MR-or CT-imaging and fascial biopsy are initiated.
In summary, the need for early diagnosis and aggressive therapy has been stressed before in the literature [7, 8, 19, 20]. We confirm this approach. Our diagnostics consisted of three steps: examination by a surgical infectious disease specialist, CT-or MR-scan and, if necessary, a biopsy, processed by rapid embedding. This allowed us to submit all patients to definitive therapy within 24 hours. In one case the diagnosis was substantiated by a biopsy prior to major surgery, in 10 cases the nature and extent of the disease was determined by CT-scan, showing a limited diagnostic validity. If anaerobes are isolated in necrotizing fasciitis an early and aggressive second look is necessary. At the same time, however, the basis of timely therapy is a high level of suspicion with no diagnostic tool being superior to clinical judgement.
- Wilson B: Necrotizing fasciitis. Am Surg 1952,18(4):416–431.PubMedGoogle Scholar
- Singh G, Sinha SK, Adhikary S, Babu KS, Ray P, Khanna SK: Necrotising infections of soft tissues--a clinical profile. Eur J Surg 2002,168(6):366–371.View ArticlePubMedGoogle Scholar
- Green RJ, Dafoe DC, Raffin TA: Necrotizing fasciitis. Chest 1996,110(1):219–229. 10.1378/chest.110.1.219View ArticlePubMedGoogle Scholar
- Giuliano A, Lewis F Jr, Hadley K, Blaisdell FW: Bacteriology of necrotizing fasciitis. Am J Surg 1977,134(1):52–57. 10.1016/0002-9610(77)90283-5View ArticlePubMedGoogle Scholar
- Chen JL, Fullerton KE, Flynn NM: Necrotizing fasciitis associated with injection drug use. Clin Infect Dis 2001,33(1):6–15. 10.1086/320874View ArticlePubMedGoogle Scholar
- Roujeau JC: Necrotizing fasciitis. Clinical criteria and risk factors. Ann Dermatol Venereol 2001,128(3 Pt 2):376–381. ReviewPubMedGoogle Scholar
- McHenry CR, Piotrowski JJ, Petrinic D, Malangoni MA: Determinants of mortality for necrotizing soft-tissue infections. Ann Surg 1995,221(5):558–563. 10.1097/00000658-199505000-00013PubMed CentralView ArticlePubMedGoogle Scholar
- Wong CH, Chang HC, Pasupathy S, Khin LW, Tan JL, Low CO: Necrotizing fasciitis: clinical presentation, microbiology, and determinants of mortality. J Bone Joint Surg Am 2003,85-A(8):1454–1460.PubMedGoogle Scholar
- Elliott DC, Kufera JA, Myers RA: Necrotizing soft tissue infections. Risk factors for mortality and strategies for management. Ann Surg 1996,224(5):672–683. 10.1097/00000658-199611000-00011PubMed CentralView ArticlePubMedGoogle Scholar
- Wong CH, Wang YS: The diagnosis of necrotizing fasciitis. Curr Opin Infect Dis 2005,18(2):101–106. Review 10.1097/01.qco.0000160896.74492.eaView ArticlePubMedGoogle Scholar
- Umbert IJ, Winkelmann RK, Oliver GF, Peters MS: Necrotizing fasciitis: a clinical, microbiologic and histopathologic study of 14 patients. J Am Acad Dermatol 1989,20(5 Pt 1):774–781.View ArticlePubMedGoogle Scholar
- Barker FG, Leppard BJ, Seal DV: Streptococcal necrotising fasciitis: comparison between histological and clinical features. J Clin Pathol 1987,40(3):335–341. 10.1136/jcp.40.3.335PubMed CentralView ArticlePubMedGoogle Scholar
- Bakleh M, Wold LE, Mandrekar JN, Harmsen WS, Dimashkieh HH, Baddour LM: Correlation of histopathologic findings with clinical outcome in necrotizing fasciitis. Clin Infect Dis 2005,40(3):410–414. 10.1086/427286View ArticlePubMedGoogle Scholar
- Wysoki MG, Santora TA, Shah RM, Friedman AC: Necrotizing fasciitis: CT Characteristics. Radiology 1997,203(3):859–863.View ArticlePubMedGoogle Scholar
- Fugitt JB, Puckett ML, Quigley MM, Kerr SM: Necrotizing fasciitis. Radiographics 2004,24(5):1472–1476. 10.1148/rg.245035169View ArticlePubMedGoogle Scholar
- Schmid MR, Kossmann T, Duewell S: Differentiation of necrotizing fasciitis and cellulitis using MR imaging. AJR Am J Roentgenol 1998,170(3):615–620.View ArticlePubMedGoogle Scholar
- Brothers TE, Tagge DU, Stutley JE, Conway WF, Del Schutte H Jr, Byrne TK: Magnetic resonance imaging differentiates between necrotizing and non-necrotizing fasciitis of the lower extremity. J Am Coll Surg 1998,187(4):416–421. 10.1016/S1072-7515(98)00192-6View ArticlePubMedGoogle Scholar
- Owens WD, Felts JA, Spitznagel EL Jr: ASA physical status classifications: a study of consistency of ratings. Anesthesiology 1978,49(4):239–243. 10.1097/00000542-197810000-00003View ArticlePubMedGoogle Scholar
- Voros D, Pissiotis C, Georgantas D, Katsaragakis S, Antoniou S, Papadimitriou J: Role of early and extensive surgery in the treatment of severe necrotizing soft tissue infection. Br J Surg 1993,80(9):1190–1191. 10.1002/bjs.1800800943View ArticlePubMedGoogle Scholar
- Ward RG, Walsh MS: Necrotizing fasciitis: 10 years experience in a district general hospital. Br J Surg 1991,78(4):488–489. 10.1002/bjs.1800780431View ArticlePubMedGoogle Scholar
- Yen ZS, Wang HP, Ma HM, Chen SC, Chen WJ: Ultrasonographic screening of clinically-suspected necrotizing fasciitis. Acad Emerg Med 2002,9(12):1448–1451. 10.1111/j.1553-2712.2002.tb01619.xView ArticlePubMedGoogle Scholar
- Majeski J, Majeski E: Necrotizing fasciitis: improved survival with early recognition by tissue biopsy and aggressive surgical treatment. South Med J 1997,90(11):1065–1068. 10.1097/00007611-199711000-00001View ArticlePubMedGoogle Scholar
- Stamenkovic I, Lew PD: Early recognition of potentially fatal necrotizing fasciitis: the use of frozen section biopsy. N Engl J Med 1984,310(26):1689–1693. 10.1056/NEJM198406283102601View ArticlePubMedGoogle Scholar
- Riseman JA, Zamboni WA, Curtis A, Graham DR, Konrad HR, Ross DS: Hyperbaric oxygen therapy for necrotizing fasciitis reduces mortality and the need for debridements. Surgery 1990,108(5):847–850.PubMedGoogle Scholar
- Brown DR, Davis NL, Lepawsky M, Cunningham J, Kortbeek J: A multicenter review of the treatment of major truncal necrotizing infections with and without hyperbaric oxygen therapy. Am J Surg 1994,167(5):485–489. 10.1016/0002-9610(94)90240-2View ArticlePubMedGoogle Scholar
- Sugihara A, Watanabe H, Oohashi M, Kato N, Murakami H, Tsukazaki S, Fujikawa K: The effect of hyperbaric oxygen therapy on the bout of treatment for soft tissue infections. J Infect 2004,48(4):330–333. 10.1016/j.jinf.2004.01.019View ArticlePubMedGoogle Scholar
- Wilkinson D, Doolette D: Hyperbaric oxygen treatment and survival from necrotizing soft tissue infection. Arch Surg 2004,139(12):1339–1345. 10.1001/archsurg.139.12.1339View ArticlePubMedGoogle Scholar
- McCord JM, Keele B-B, Fridovich I: An enzyme-based theory of obligate anaerobiosis: the physiological function of superoxide dismutase. Proc Natl Acad Sci USA 1971,68(5):1024–1027. 10.1073/pnas.68.5.1024PubMed CentralView ArticlePubMedGoogle Scholar
- Wolters U, Wolf T, Stützer H, Schröder T: ASA classification and perioperative variables as predictors of postoperative outcome. Br J Anaesth 1996,77(2):217–222.View ArticlePubMedGoogle Scholar
- Klotz HP, Candinas D, Platz A, Horvàth A, Dindo D, Schlumpf R, Largiadèr F: Preoperative risk assessment in elective general surgery. Br J Surg 1996,83(12):1788–1791. 10.1002/bjs.1800831240View ArticlePubMedGoogle Scholar