Volume 14 Supplement 4
Angiotensin-converting enzyme I/D polymorphism in chronic obstructive pulmonary disease
© I. Holzapfel Publishers 2009
Published: 7 December 2009
The etiology of chronic obstructive lung disease (COPD) is unclear. It is supposed to be the product of an exogenous antigenic stimulus, such as tobacco smoke, and an endogenous genetic susceptibility. The angiotensin-converting enzyme (ACE) gene contains a polymorphism based on the presence (insertion [I]) or absence (deletion [D]) of a 287-bp nonsense domain, resulting in three different genotypes (II, ID and DD). The aim of the study was to find out whether the ACE gene polymorphism can determine the course of COPD.
Patients and design
We genotyped 152 Caucasian patients with COPD and 158 healthy controls for the ACE (I/D) polymorphism. We divided the COPD group into one group of 64 patients with a stable course of disease, defined as less than three hospitalizations over the last three years due to COPD, and another group of 88 patients with an instable course with more than three hospitalizations.
The I-allele was significantly associated with an increased risk for COPD in a dominant model (OR 1.67 (95% CI 1.00 to 2.78), p = 0.048), but not in a recessive or co-dominant model. Moreover, the I-allele of ACE (I/D) was significantly increased in patients with a stable course of COPD (p = 0.012) compared with controls. In a dominant model (II/ID v DD) we found an even stronger association between the I-allele and a stable course of COPD (OR 3.24 (95% CI 1.44 to 7.31), p = 0.003).
These data suggest that the presence of an ACE I-allele determines a stable course of COPD.
KeywordsCOPD angiotensin converting enzyme (ACE) genetics
Airway inflammation is the main pathological feature of patients with chronic obstructive lung disease (COPD). Chronic bronchitis leads to destruction of alveoli and finally ends in irreversible lung emphysema. Exacerbations of COPD are defined as an acute onset of worsening of the patient's condition, often caused by bacterial infection [1–3]. The most important exogenous risk factor for developing a COPD is inhalant tobacco smoke. However, only approximately 20% of long term smokers develop a COPD, indicating that other factors are at play. Experimental studies in mice have shown that an interindividual susceptibility leads to a different phenotype after tobacco smoke exposure . A genetic background is supported by family studies . A monogenic susceptibility such as α 1-antitrypsin deficiency accounts only for a minority of patients with COPD. These facts suggest the existence of a polygenic fixed susceptibility. Despite the advances made in the therapeutic approach, the basic mechanisms of the pathogenesis are still poorly understood. Recently, efforts to elucidate the genetic background of COPD have been made in an increasing fashion. In the meantime different polymorphisms in potential candidate genes for the development or course of COPD have been detected. For instance, as shown for bronchial asthma there is evidence for an association between IL-13 polymorphisms and the onset of COPD . Polymorphisms in matrix-metalloproteases (MMP)-1 and 12 were linked to a rapid progressive course . Ito et al. (8) pointed out that polymorphisms in MMP-9 are associated with the location of lung emphysema in patients with COPD . However, there is less evidence which genes take part in the progression of the disease i.e. which genes codetermine the rate of exacerbation and deterioration of lung function.
Angiotensin-converting enzyme (ACE) plays an important role in circulatory homeostasis. In this context ACE exerts tonic influence on water balance and blood pressure. The ACE gene contains a polymorphism based on the presence (insertion [I]) or absence (deletion [D]) within an intron of a 287-bp nonsense domain, resulting in three different genotypes (II, ID and DD) . The ACE DD genotype is associated with increased cellular and circulating concentrations of ACE . There is evidence that lower ACE activity may have benefit in long term course of patients with COPD , but the mechanisms are still poorly understood. As ACE-mediated pathogenic factors may be involved in the pathogenesis of COPD, we examined the ACE I/D polymorphism in 152 patients with chronic obstructive lung disease and 158 healthy control subjects.
Materials and Methods
The research was carried out in accordance with the Declaration of Helsinki (1989) of the World Medical Association, and the study was approved by the Ethics Committee of Bonn University School of Medicine, Germany and of St. George Medical Center in Leipzig, Germany. Written informed consent was obtained from each patient prior to their enrollment. Patients with severe medical disorders including malignancy and immunological disorders were excluded from the study. All patients were at least 18 old.
Blood samples were collected from two groups of patients with COPD. The diagnosis of chronic obstructive lung disease was made according to the guidelines of the ATS/ERS . The first group comprised 88 patients admitted to Medical Clinic and Policlinic II, Department of Medicine, Bonn University Hospital, Germany and the Department of Internal Medicine, St. George Medical Center in Leipzig, Germany. These patients enrolled to the first study had more than 3 hospitalizations over the last three years due to exacerbations of COPD. These patients have been defined as instable. Lung function in these subjects was measured before discharge. The second group included 64 outpatients with stable COPD treated in the same hospitals. Body plethysmographic tests were performed according to the ATS/ERS criteria .
A third group (158 healthy controls) was selected in pre-engaging examinations at Bonn University, Germany. They were all residents of Germany. None had a history of lung disease or showed any symptoms of lung or other disease. Lung function tests were performed in all controls. All showed normal findings in laboratory examination, which included complete blood counts, urine analyses, hepatic enzyme activities and BUN levels.
Smoking habit was defined as follows: non-smokers had never smoked; ex-smokers had smoked daily and given it up prior to entering the study. Smokers have smoked daily at the time of study. The amount of lifetime smoking was assessed as pack years (years of smoking x number of packs of 20 cigarettes per day).
Peripheral venous blood samples of 9 ml were drawn from each patient by standard venous puncture. Each blood sample was collected in sterile tubes containing 15% K3EDTA solution. DNA was isolated by salting out procedure described by Miller et al. .
Determination of the ACE Genotype
Sequences of primers as used for PCR.
control PCR for detection of I, if DD in first PCR
2, 5 mM
All statistical analysis was performed using a statistical software package (SPSS v. 13.0; SPSS; Chicago, IL). Demographic data of patients having ACE gene polymorphisms were compared using a one-way analysis of variance. Differences in the frequencies of alleles and genotypes between patients and control subjects were tested by the χ2 test (Pearson's goodness-of-fit and Armitage's trend test) .
Baseline characteristics of patients with COPD and healthy control subjects (means ± SD).
COPD all patients
Statistical analysis of the case-control study.
Dominant (II/ID v DD)
Recessive (II v ID/DD)
To exclude an influence of age, sex or smoking habit we performed a regression analysis, where we could not see any impact of these factors on the results. Taken together, there is a significant association between the prevalence of ACE-I-allele and a stable course of COPD.
We here demonstrate that ACE I-allele is associated with a stable course in COPD-patients. The ACE Dallele is less common in outpatients with fewer hospital admissions due to COPD compared with healthy controls and with patients with an instable course of COPD. Although we could show a significant association between COPD and the I-allele in a dominant model, we propose that ACE gene might not be a susceptibility gene for the onset of COPD but a disease modifying gene.
Endocrine (or circulating) angiotensin converting enzyme plays an important role in the renin-angiotensin-aldosterone-system (RAAS), which influences circulatory homeostasis by salt and water retention. Moreover, a cellular (autocrine) and organ (paracrine) RAAS in different tissues exists. In both systems the presence (insertion, I allele) or the absence (deletion, D allele) of a 287 base pair nonsense DNA domain within an intron in the human ACE gene cause three different genotypes: II, ID an DD. The D allele is associated with a higher ACE serum level and therefore an increased activity. Activated RAAS mediates distinct physiological effects caused by angiotensin II and aldosterone. In all groups studied, ACE polymorphisms were in Hardy-Weinberg-equilibrium. The distribution of ACE genotypes in the control group of the present study did not significantly differ from those described in control populations of other studies [17–20].
There is evidence that lower ACE activity may have benefit in long term course of patients with COPD . However, the mechanisms leading to this observation are still unclear. One possible explanation could be potential effects on pulmonary inflammation. Powerful proinflammatory effects in different models and target tissues have been described. For example, DD genotype is increased in patients with acute adult respiratory distress syndrome (ARDS) and is also associated with a higher mortality in the ARDS group . Another reason for the clinically observed more stable course of non-D-Allele could be due to the fact that the DD allele has been shown to be negatively associated with right ventricular hypertrophy in male COPD patients . Right ventricular hypertrophy is an important co-morbidity of COPD that contributes to predict mortality . Moreover, right ventricular decompensation with elevated serum troponin, which is found in 20% of patients with acute exacerbations of COPD, are independent predictors of in-hospital mortality .
Another reason for the more benign course of COPD associated with non-D-allele could be due to modified muscle architecture described in different ACE-genotypes. The presence of ACE-II-allele confers an enhanced mechanical efficiency in trained muscle  in a general population. Furthermore, it could be shown that ACE-II-polymorphism may conserve a positive energy balance during rigorous training, which suggests enhanced metabolic efficiency . Also in COPD patients it could be demonstrated that I, and not D, was associated with an enhanced response to physical training . In different to Caucasian ethnicities, COPD patients from China did not show any correlation between ACE-I/D polymorphisms and ventilatory response in cardiopulmonary exercise testing . These facts taken together could lead to the assumption that trained breathing musculature in COPD patients with non-DD-allele works more efficient even in oncoming exacerbations with higher work of breathing and less oxygen saturation. The actual hospitalizations therefore could sometimes be averted by an enhanced breathing capacity.
The ACE DD genotype may also be associated with impairment in peripheral tissue oxygenation during exercise in patients with COPD . The worsening of hypoxemia and gas exchange in acute exacerbations of COPD perhaps can be explained by increased ventilation/perfusion inequality and this effect is amplified by a decrease of mixed venous oxygen tension that results from greater oxygen consumption, presumably because of increased work of the respiratory muscles . Summing up, it may be said that there probably is a comprehensive background that leads to the clinical observation we made. Nevertheless, determination of ACE(I/D) polymorphism could help us to predict the course of COPD in the future and therefore may have an influence on the treatment and surveillance of COPD patients.
In conclusion, this study shows an association between the ACE I allele and a stable course of COPD with less hospital admissions. Future studies with a larger number of patients should extend the findings of this study. It would be of interest whether depressing the RAAS by ACE-inhibitors or angiotensin II antagonists could contribute to a slower progress and stability in COPD-patients.
Conflicts of interest
There was not any personal or financial support or author involvement with organizations with financial interest in the subject matter and that effect concerns all the authors of this article.
Chronic Obstructive Pulmonary Disease
polymerase chain reaction
The authors wish to thank all patients in the present study for their participation. Especially we thank the staff of the participating hospitals that helped in patient recruitment and sample collection. We would also like to thank Prof. T. Wienker of the Department of Genetic Epidemiology, Bonn University for his help in statistical analysis. This study was supported by institutional grants (BONFOR).
- Burge S, Wedzicha JA: COPD exacerbations: definitions and classifications. Eur Respir J Suppl 2003, 41: 46s-53s.PubMedView ArticleGoogle Scholar
- Garcia-Aymerich J, Monsó E, Marrades RM, Escarrabill J, Félez MA, Sunyer J, Antó JM: EFRAM Investigators. Risk factors for hospitalization for a chronic obstructive pulmonary disease exacerbation. EFRAM study. Am J Respir Crit Care Med 2001, 164: 1002–7.PubMedView ArticleGoogle Scholar
- Donaldson GC, Seemungal TA, Bhowmik A, Wedzicha JA: Relationship between exacerbation frequency and lung function decline in chronic obstructive pulmonary disease. Thorax 2002, 57: 847–52. 10.1136/thorax.57.10.847PubMed CentralPubMedView ArticleGoogle Scholar
- Guerassimov A, Hoshino Y, Takubo Y: The development of lung emphysema in cigarette-exposed mice is strain dependant. Am J Respir Crit Med 2004, 170: 974–80. 10.1164/rccm.200309-1270OCView ArticleGoogle Scholar
- McCloskey SC, Patel BD, Hinchliffe SJ, Reid ED, Wareham NJ, Lomas DA: Siblings of patients with severe chronic obstructive pulmonary disease have a significant risk of airflow obstruction. Am J Respir Cit Care Med 2001, 164: 1419–24.View ArticleGoogle Scholar
- van der Pouw Kraan TC, Küçükaycan M, Bakker AM, Baggen JM, van der Zee JS, Dentener MA, Wouters EF, Verweij CL: Chronic obstructive pulmonary disease is associated with the 1055 IL-13 promoter polymorphism. Genes Immun 2002, 3: 436–9. 10.1038/sj.gene.6363896PubMedView ArticleGoogle Scholar
- Joos L, He JQ, Shepherdson MB, Connett JE, Anthonisen NR, Paré PD, Sandford AJ: The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum Mol Genet 2002, 11: 569–76. 10.1093/hmg/11.5.569PubMedView ArticleGoogle Scholar
- Ito I, Nagai S, Handa T, Muro S, Hirai T, Tsukino M, Mishima M: Matrix metalloproteinase-9 promoter polymorphisms associated with upper lung dominant emphysema. Am J Respir Crit Care Med 2005, 172: 1378–82. 10.1164/rccm.200506-953OCPubMedView ArticleGoogle Scholar
- Rigat B, Hubert C, Corvol P, Soubrier F: PCR detection of the insertion/deletion polymorphism of the human angiotensin converting enzyme gene (DCP1) (dipeptyl carboxy peptidase 1). Nucl Acids Res 1992, 20: 1433.PubMed CentralPubMedView ArticleGoogle Scholar
- Rigat B, Hubert C, Alhenc-Gelas F, Cambien F, Corvol P, Soubrier F: An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest 1990, 86: 1342–6.View ArticleGoogle Scholar
- Forth R, Montgomery H: ACE in COPD: a therapeutic target? Thorax 2003, 58: 556–8. 10.1136/thorax.58.7.556PubMed CentralPubMedView ArticleGoogle Scholar
- Celli BR, MacNee W, ATS/ERS Task force: Standards for the diagnosis and treatment of patients with COPD: a summary of the ATS/ERS position paper. Eur Respir J 2004, 23: 932–46. 10.1183/09031936.04.00014304PubMedView ArticleGoogle Scholar
- Pellegrino R, Viegi G, Brusasco V, Crapo RO, Burgos F, Casaburi R, Coates A, van der Grinten CP, Gustafsson P, Hankinson J, Jensen R, Johnson DC, MacIntyre N, McKay R, Miller MR, Navajas D, Pedersen OF, Wanger J: Interpretive strategies for lung function tests. Eur Respir J 2005, 26: 948–68. 10.1183/09031936.05.00035205PubMedView ArticleGoogle Scholar
- Miller SA, Dykes DD, Polesky HF: A simple salting out procedure for extracting DNA from human nucleated cells. Nucl Acids Res 1988, 16: 1215. 10.1093/nar/16.3.1215PubMed CentralPubMedView ArticleGoogle Scholar
- Ruprecht B, Schürmann M, Ziegenhagen MW, vom Bauer E, Meier D, Schlaak M, Müller-Quernheim J: Corrected normal values for serum ACE by genotyping the deletion/insertion-polymorphism of the ACE-gene. Pneumologie 2001, 55: 326–32. 10.1055/s-2001-15616PubMedView ArticleGoogle Scholar
- Sasieni PD: From genotypes to genes: Doubling the sample size. Biometrics 1997, 53: 1253–61. 10.2307/2533494PubMedView ArticleGoogle Scholar
- Cambien F, Poirier O, Lecerf L, et al.: Deletion polymorphism in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction. Nature 1992, 359: 641–4. 10.1038/359641a0PubMedView ArticleGoogle Scholar
- van Suylen RJ, Wouters EF, Pennings HJ, Cheriex EC, van Pol PE, Ambergen AW, Vermelis AM, Daemen MJ: The DD genotype of the angiotensin converting enzyme gene is negatively associated with right ventricular hypertrophy in male patients with chronic obstructive lung disease. Am J Respir Crit Care Med 1999, 159: 1791–95.PubMedView ArticleGoogle Scholar
- Hopkinson NS, Nickol AH, Payne J, Hawe E, Man WD, Moxham J, Montgomery H, Polkey MI: Angiotensin converting enzyme genotype and strength in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 2004, 170: 395–9. 10.1164/rccm.200304-578OCPubMedView ArticleGoogle Scholar
- Gosker HR, Pennings HJ, Schols AMWJ: ACE gene polymorphism and COPD. Am J Respir Crit Care Med 2004, 170: 572.PubMedView ArticleGoogle Scholar
- Marshall RP, Webb S, Bellingan GJ, Montgomery HE, Chaudhari B, McAnulty RJ, Humphries SE, Hill MR, Laurent GJ: Angiotensin converting enzyme insertion/deletion polymorphism is associated with susceptibility and outcome in acute respiratory distress syndrome. Am J Respir Crit Care Med 2002, 166: 633–4. 10.1164/rccm.2206004View ArticleGoogle Scholar
- Antonelli Incalzi R, Fuso L, De Rosa M, Forastiere F, Rapiti E, Nardecchia B, Pistelli R: Co-morbidity contributes to predict mortality of patients with chronic obstructive lung disease. Eur Respir J 1997, 10: 2794–800. 10.1183/09031936.97.10122794PubMedView ArticleGoogle Scholar
- Baillard C, Boussarsar M, Fosse JP, Girou E, Le Toumelin P, Cracco C, Jaber S, Cohen Y, Brochard L: Cardiac troponin I in patients with severe exacerbations of chronic obstructive pulmonary disease. Intensive Care Med 2003, 19: 584–9.Google Scholar
- Williams AG, Rayson MP, Jubb M, World M, Woods DR, Hayward M, Martin J, Humphries SE, Montgomery HE: The ACE gene and muscle performance. Nature 2000, 403: 614.PubMedView ArticleGoogle Scholar
- Montgomery H, Clarkson P, Barnard M, Bell J, Brynes A, Dollery C, Hajnal J, Hemingway H, Mercer D, Jarman P, Marshall R, Prasad K, Rayson M, Saeed N, Talmud P, Thomas L, Jubb M, World M, Humphries S: Angiotensin-converting-enzyme gene insertion/deletion polymorphism and response to physical training. Lancet 1999, 353: 541–5. 10.1016/S0140-6736(98)07131-1PubMedView ArticleGoogle Scholar
- Zhang X, Wang C, Dai H, Lin Y, Zhang J: Association between angiotensin-converting enzyme gene polymorphisms and exercise performance in patients with COPD. Respirology 2008, 13: 683–8. 10.1111/j.1440-1843.2008.01325.xPubMedView ArticleGoogle Scholar
- Kanazawa H, Otsuka T, Jirata K, Yoshikawa J: Association between the angiotensin-converting enzyme gene polymorphisms and tissue oxygenation during exercise in patients with COPD. Chest 2002, 121: 697–701. 10.1378/chest.121.3.697PubMedView ArticleGoogle Scholar
- Barberà JA, Roca J, Ferrer A, Félez MA, Díaz O, Roger N, Rodriguez-Roisin R: Mechanisms of worsening gas exchange during acute exacerbations of chronic obstructive pulmonary disease. Eur Respir J 1997, 10: 1285–91. 10.1183/09031936.97.10061285PubMedView ArticleGoogle Scholar