Safety and efficacy after switch to a saquinavir-containing antiretroviral regimen in protease inhibitor pretreated HIV-positive patients
© I. Holzapfel Publishers 2010
Received: 21 September 2009
Accepted: 5 January 2010
Published: 24 September 2010
The RAINBOW survey is a multinational observational study assessing the tolerability and efficacy of ritonavir-boosted saquinavir (SQV/r), using the 500 mg film-coated SQV formulation, in routine clinical practice. This analysis presents data from the German subgroup of protease inhibitor (PI)-pretreated, but SQV-naïve patients.
Multicenter, prospective, open-label, 48 week cohort study. Efficacy assessments included the proportion of patients with HIV-1 RNA < 50 and < 400 copies/mL and changes in CD4 cell count from baseline to week 48. Tolerability assessments included changes in liver enzymes and lipid levels from baseline to week 48.
A total of 426 patients were included in the analysis. The proportion of patients with HIV RNA levels < 50 copies/mL at week 48 was 60.3% (compared with 31.7% at switch to SQV/r) (intent-to-treat, last observation carried forward analysis). After 48 weeks, median CD4 count increased by +61 cells/mm3 from baseline (p < 0.01) and 60.3% of patients achieved HIV-1 RNA < 50 copies/mL. Median changes in fasting triglyceride levels (stratified according to baseline level) at week 48 were: +14 mg/dL (IQR -8; 57) for patients with baseline triglyceride < 200 mg/dL; -50 mg/dL (IQR -139; 0) for baseline triglyceride 200-750 mg/dL, and -656 mg/dL (IQR 1024; 0) for baseline triglyceride > 750 mg/dL (p < 0.01 for all). Median changes in fasting total cholesterol (TC) levels (stratified according to baseline) were +16 mg/dL (IQR -3; 43) for patients with baseline TC < 200 mg/dL (p < 0.01), -3 mg/dL (IQR -25; 25) for baseline TC 200-300 mg/dL (p = 0.4), and -47 mg/dL (IQR -87; -4) for baseline TC > 300 mg/dL (p < 0.01). No significant changes in liver enzymes or bilirubin were observed. SQV treatment was discontinued in 22% of patients, 6% due to side effects.
These data confirm the efficacy and tolerability of SQV/r in PI-experienced, SQV-naïve patients treated in a real-life clinical setting. Of particular relevance are the improvements in triglycerides and TC levels observed in patients with baseline grade III-IV elevations.
KeywordsHIV protease inhibitor saquinavir cholesterol triglycerides
The development of metabolic alterations such as dyslipidemia and body fat changes (lipodystrophy) is a major consideration with the use of protease inhibitors (PIs) as part of Highly Active Anti-Retroviral Therapy (HAART) . The link between HAART and increased risk of cardiovascular disease was confirmed almost a decade ago , and increased exposure to PIs in particular has been associated with an increased risk of myocardial infarction, which is partly explained by dyslipidemia . Treatment with PIs is associated with significantly increased levels of triglycerides, total cholesterol (TC), low-density lipoproteins (LDL) and high-density lipoproteins (HDL), as well as changes in body fat . Lipid alterations and morphologic abnormalities vary between the different PIs; for example, indinavir has been associated with insulin resistance , and ritonavir-boosted lopinavir with hypertriglyceridemia and hypercholesterolemia , whereas atazanavir has been reported to have a relatively favorable lipid profile . There are also differences in the reported efficacy and tolerability of PIs and therefore selecting and switching treatment regimens in those patients with virological failure, toxicity, or changes in lipid profiles related to a particular PI represents an important management issue.
Saquinavir (Invirase®, SQV) is a potent inhibitor of HIV-1 viral protease, which, when co-administered with ritonavir boosting (SQV/r) has been shown to be effective in clinical trials of treatment-naïve and treatment-experienced patients and to be associated with a more favorable lipid profile than ritonavir-boosted lopinavir and indinavir, with improved tolerability [8–11]. Ritonavir-boosted SQV is currently recommended for initial PI-based ART by international HIV treatment guidelines [12–15]. Initially available as 200 mg hard capsules, SQV is now available as a 500 mg film-coated tablet formulation which achieves the current recommended daily dose (1000 mg twice daily with 100 mg ritonavir twice daily ) with a reduced pill burden and simplified dosing . Although there is currently wide treatment experience with various SQV/ritonavir combinations and with the previous SQV formulations, available data on the 500 mg film-coated tablets is yet limited.
The international RAINBOW survey was designed to identify a cohort of HIV-1 infected patients who initiated treatment with, 500 mg film-coated SQV-tablet in routine clinical practice. The survey aimed to include a wide cross-section of patient populations, including ART-naïve patients and ART-experienced patients with a range of pretreatment histories. In this analysis we present the final 48-week efficacy and tolerability data from patients who were previously treated with one or more PIs (PI-experienced), but who were SQV- naïve, from the German RAINBOW COHORT.
Materials and methods
The RAINBOW COHORT was a multicenter, prospective, non-interventional 48 week observational study in HIV-patients treated with SQV 500 mg film-coated tablets as part of their antiretroviral regimen and took place between July 2005 and May 2008.
Inclusion criteria for RAINBOW were documented HIV-1 infection and initiation of treatment with SQV/ritonavir (SQV/r) utilizing the 500 mg film-coated SQV tablet, in combination with other antiretrovirals as part of routine antiretroviral therapy (ART).
Patient selection for SQV-based antiretroviral treatment was at the discretion of the treating physician, and the decision was made in accordance with the declaration of Helsinki and with German treatment guidelines, published by the German AIDS-society . Patients were included in the RAINBOW COHORT if they had been previously treated with PI containing regimens, but that did not include SQV. There were no specified exclusion criteria, and no restrictions on the use of concomitant medication. In order to avoid selection bias, all investigators were encouraged to report data from all eligible patients at their site rather than reporting on a random subset only. For patients who discontinued SQV/r treatment or with missing data, the last recorded values were carried forward for inclusion in the final analysis (up to week 48).
The following demographic/baseline characteristic data were collected: date of birth; ethnicity; gender; height; weight; previous diseases; HIV risk exposure; year of diagnosis; presumed mode of infection; current disease stage; HIV-RNA viral load and CD4+ cell count prior to starting boosted SQV treatment; antiretroviral drug history; concomitant medications, including any lipid lowering and antidiarrhetic medications; and hematology and biochemistry laboratory results, including blood where available.
Efficacy and tolerability data were collected from the patient records and recorded on a central database using identifying numbers only. Patient visits were allocated to applicable windows: week 2-6, week 8-16, week 20-28, week 32-40, and week 44-52. The following data were recorded from the patient charts: CD4+ cell count; HIV-RNA viral load; date of HIV-RNA viral load and CD4+ cell count; HIV-RNA assay used; lower limit of HIV-RNA assay used; weight; changes in background antiretroviral drugs; changes to/introduction of lipid lowering medications, antidiarrhetic medication and other concomitant medication; unexpected clinical adverse events; treatment adherence information and reason of premature withdrawal; hematology and biochemistry laboratory results (including fasting blood lipids).
Tolerability endpoints were: changes in fasting lipids levels (total cholesterol [TC], high-density lipoprotein [HDL] and low-density lipoprotein [LDL] cholesterol, and triglycerides) from baseline to week 48 stratified according to baseline levels; changes in liver enzymes (alanine aminotransferase [ALT], aspartame aminotransferase [AST], and gamma glutamyl transferase [GGT]); and frequency of Grade 3 and 4 adverse events or death, including those considered by the treating physician to be related to SQV.
Efficacy endpoints were: percentage of patients with HIV-RNA < 50 copies/mL at week 48; percentage of patients with HIV-RNA < 400 copies/mL at week 48; and change in CD4 cell count from baseline at week 48.
All PI-experienced, SQV-naïve patients included in the RAINBOW COHORT with complete baseline visit were included in the analysis. Reflecting the observational nature of the study, we used an intent-to-treat (ITT) analysis of last observation carried forward (LOCF) i.e. missing values for HIV-RNA and CD4+ cell count values, lipids and liver enzymes were taken as the previous observed value.
Descriptive summary statistics were used for this cohort analysis, characterizing changes in CD4+ cell count and HIV-RNA from baseline, the type and frequency of adverse events observed, and changes in safety relevant laboratory parameters. For continuous variables the following statistics were calculated: mean; standard deviation, median, inter-quartile range (IQR) and minimum and maximum values. For categorical variables, number of values in each category and percentage of the values with regard to number of patients in the study population were calculated. Explorative statistical methods were used with regard to the efficacy endpoints and changes in safety-relevant laboratory parameters. Changes from baseline were tested for significance using the Wilcoxon signed rank test. Shifts in ranking of baseline values and week 48 values were assessed using Shift tables and McNemar/Bowker tests.
Baseline characteristics and demographics
PI-experienced, but SQV-naïve patients
n = 426
Male, % (n)
Age in years, median (IQR)
43 (37; 49)
Height in cm, median, (IQR)
177 (172; 182)
weight in kg, median (IQR)
72 (65; 80)
Hepatitis b, % (n)
Hepatitis c, % (n)
Infection mode, %
CDC stage, %
Time since first diagnosis in years, median (IQR)
9 (4; 14)
Time since first ART in years, median (IQR)
7.3 (3.4; 9.4)
Number of previous regimen, median (IQR)
4 (3; ≥ 6)
Number of previous PIs, median (IQR)
2 (1; 3)
Treatment interruption before start of new regimen, % (n)
Duration of treatment interruption in months, median (IQR)
8.8 (3; 23)
HIV RNA (copies/ml), median (IQR)
599 (< 50; 31,291)
Proportion of patients with HIV RNA < 50 copies/ml at baseline
CD4-count (cells/mm3) median (IQR)
320 (177; 506)
Previous antiretroviral regimen
≥ 3 NRTI
NNRTI + NRTI
PI/r + NRTI/NNRTI
Antiviral drugs included in SQV/ritonavir regimens NRTI: nucleoside reverse transcriptase inhibitor; NNRTI: non-nucleoside reverse transcriptase inhibitors; PI: protease inhibitor; TDF: tenofovir; FTC: emtricitabine; AZT: zidovudine; 3TC: lamivudine; ABC: abacavir; LPV: lopinavir; ATV: atazanavir; FAP: fosamprenavir
PI + NRTI/NNRTI
The majority of patients received SQV/r either as part of a PI plus two NRTIs regimen, most commonly TDF and FTC, or as a double PI regimen, most commonly with LPV.
In total, 22% (94/426) of the patients withdrew from the study prior to week 48 (3.3% by week 4; 4.2% week 4-12; 6.3% week 12-24; 4.0% week 24-36; 4.2% week 36-48). Reasons for discontinuations were: side effects (5.5%; n = 24), virological failure (3.5%; n = 15), lost to follow up (4%; n = 18), lack of adherence (3.5%; n = 15), intercurrent disease (1%; n = 3) and death (0.5%; n = 2; all classified as unrelated to SQV). Reasons discontinuations were not specified for 17 patients (4%). Of the 15 patients who withdrew from study due to virological failure, resistance data prior to the switch to the SQV-based regimen were available for eight patients, and resistance against a median of 3 (0; 6) protease inhibitors was documented. No resistance data were available for the remaining seven patients.
Safety and tolerability
Adverse events (AEs) related to SQV
Number of AEs
Number of patients
AEs Grade 3/4
There were no clinically relevant changes from baseline in liver enzyme or bilirubin levels at week 48. Median changes from baseline to week 48 in female patients were: ALT -3.0 U/L (IQR -10.0, 4.0; n = 55); AST -2.0 U/L (IQR -7.0, 6.0; n = 47); GGT 5.0 U/L (IQR -2, 45.6; n = 54) (ITT, LOCF analysis). In male patients, median changes from baseline to week 48 were: ALT 0.0 U/L (IQR -8.0, 6.0; n = 335); AST 0.0 U/L (IQR -6.0, 5.0; n = 301); GGT 8.15 U/L (IQR -0.55, 393; n = 332) (ITT, LOCF analysis). Median change in bilirubin levels from baseline to week 48 was 0.0 mg/dl (IQR -0.2, 0.25; n = 258).
Virological and immunological response
Median increase in CD4 count was + 61 cells/mm3 (IQR -9; 170; p < 0.01) from baseline to week 48 (Figure 3). The proportion of patients achieving HIV RNA levels of < 50 copies/mL by 48 weeks was 60.3% (compared with 31.7% at baseline) and 74.9% (compared with 44.8% at baseline) for < 400 copies/mL (ITT, LOCF analysis), respectively (Figure 3).
In patients who switched to SQV because of toxicity to another PI, HIV-RNA and CD4 levels remained stable. For those patients who switched to SQV because of virological failure, there was a marked improvement in these parameters.
Changes in lipid profiles
With regard to LDL levels, although in the cohort of patients as a whole there was a significant shift towards an increase in LDL levels between baseline and week 48 in the McNemar/Bowker test (p = 0.01), only patients with LDL < 130 mg/dL at baseline showed a small increase in LDL cholesterol at week 48 (+8 mg/dL [IQR -4; 27]; p < 0.01; n = 146) There was no overall change in levels for patients with LDL 130-190 mg/dL at baseline (0 mg/dL [IQR -15; 14]; p = 0.79; n = 66), while levels fell significantly in patients with LDL > 190 mg/dL at baseline (-41 mg/dL [IQR -49; 6]; p < 0.05; n = 9) (ITT, LOCF analysis) (Figure 2a).
There was also a small, but non-significant increase in HDL levels between baseline and week 48 in the group of patients as a whole by the Bowker test (p = 0.13) (data not shown). When stratified according to baseline, this was significant in patients with HDL ≥ 40 mg/dl at baseline (+ 5 mg/dL [IQR 0; 9]; p = 0.01; n = 93).
The proportion of patients receiving lipid lowering agents remained stable throughout the study period (6.6% at baseline vs. 6.1%, 6.3%, 5.9% and 6.3% at weeks 12, 24, 36 and 48, respectively).
The following parameters did not affect outcome in terms of lipid levels: use of lipid-lowering agents; previous treatment with stavudine or efavirenz; previous therapy using double PIs; new use of SQV in double PI therapy.
This analysis confirms that ritonavir-boosted SQV is effective and well-tolerated in PI-experienced but SQV-naïve patients in a real-life clinical setting. Our data demonstrate that switching to SQV was beneficial both for those patients who had intolerance or toxicity to their previous ART regimen, and those who failed previous treatment due to virological failure. The proportion of patients with HIV RNA levels < 50 copies/mL at 48 weeks was almost twice that seen at baseline, and a significant increase in CD4 cell count was also seen by 48 weeks. Interestingly, the increase in CD4 count reflected the virological response. Increases in CD4 count and reductions in HIV-1 RNA levels were especially marked in those patients who switched to SQV because of previous virological failure. In PI-experienced patients with Grade 3/4 elevations in lipid levels at baseline, switching treatment to SQV was associated with significant and marked improvements in triglyceride, LDL cholesterol and TC levels. Overall, lipid level changes after 48 weeks of SQV-based ART were relatively small in those patients with normal baseline levels, but median levels were significantly improved in those patients with Grade 3/4 levels at baseline. Of note, median triglyceride levels in PI-pretreated patients with Grade 3/4 levels showed a particularly marked decline. The favorable triglyceride, lipid and liver enzyme and bilirubin profiles confirm the tolerability of SQV 500 mg film tablets in this group of patients.
The tolerability and efficacy of SQV/r 1000/100 mg bid has been demonstrated in the a number of trials, which incorporated a range of patient types including patients who switched to SQV/r as a result of intolerance or virological failure [8, 12, 18, 19]. These studies predominantly used the earlier 200 mg hard-capsule or soft gel formulations of SQV. The 500 mg film-coated formulation of SQV used in our study was introduced to address the problems of high pill burden and its potential effect on adherence . As there are currently few data from studies using the new formulation in PI-experienced patients, we conducted the current analysis to investigate the safety and efficacy of the 500 mg film-coated formulation of SQV in this patient population treated in a real-life clinical setting. Interestingly, results from our cohort of PI-experienced patients are similar to those seen in ART-naïve patients. In the open-label, non-inferiority GEMINI trial of first-line SQV/r plus emtricitabine/tenefovir (FTC/TDF) vs. ritonavir-bosted lopinavir (LPV/r) plus FTC/TDF, 64.7% and 72.5% of patients in the SQV/r arm achieved HIV-1 RNA levels < 50 copies/mL and < 400 copies/mL, respectively , compared with 60.3% and 74.9%, respectively, in the current analysis.
The incidence and nature of AEs were similar in our cohort of patients to those previously reported in clinical trials and were most commonly gastrointestinal and diarrhea [8, 9, 11, 18]. There was no indication of relevant changes in liver enzymes or bilirubin in our patient cohort. Changes in lipid profile are of particular concern with PI-based ART, with treatment-related dyslipidemia being linked to atherogenesis and increased risk of coronary artery disease [1–4]. Treatment with SQV/r has been found to be associated with a more favorable lipid profile than ritonavirboosted indinavir and lopinavir [8, 11]. In the current analysis, switching to SQV/r resulted in a marked decrease in levels of both triglycerides and total cholesterol in those patients who had increased baseline levels, most markedly in those with Grade III/IV levels at baseline. Furthermore, LDL cholesterol levels also declined in those patients with high baseline levels in our analysis. Despite a slight increase in median triglyceride-, total cholesterol- and LDL cholesterol-levels in patients within the normal range at baseline, the whole proportion of patients within the normal range for these parameters was comparable high, pre- and post-SQV/r therapy. Taken together, these finding suggest a favorable lipid profile of SQV/r for PI-experienced patients.
The main strength of the current analysis of patients from the RAINBOW survey lies in the fact that it consists of an unselected cohort of HIV-infected patients treated under routine, 'real-life' conditions in contrast to the highly selective and controlled randomised trial situation. The data derived from such cohorts may more closely reflect clinical practice than results obtained from the optimized setting of clinical studies. However the study has the usual limitations of observational analyses. It is not possible to rule out selection bias, although all RAINBOW investigators were encouraged to report data from all eligible patients at their site rather than reporting on a random subset only. In addition, the case-payment system used for data collection was based on completed visits per case, which may also have encouraged reporting.
This cohort study adds to the efficacy and safety data available for the new formulation of SQV used at the recommended treatment dose with ritonavir boosting by investigating its tolerability and effectiveness in PI-experienced patients. In addition it confirms the efficacy and tolerability of SQV seen in clinical trials in the 'real-world' clinical setting. Based on the findings of this analysis, SQV/r offers a viable option both for patients who have virological failure on previous PI-based therapy, or who suffer toxicity. In particular, in those patients with markedly elevated lipid levels following PI treatment, a switch to SQV/r was associated with a marked decline in triglyceride, total cholesterol and LDL cholesterol.
In conclusion, results from this observational cohort of PI-experienced patients treated with the currently approved dose of SQV/r and using the 500 mg film-coated SQV formulation in the 'real life' clinical setting are consistent with high efficacy and tolerability seen in controlled studies with SQV/r.
The authors would like to thank all centers participating in German RAINBOW COHORT: Bänsch U, Berlin; Baumann R, Neuss; Becker W, München; Bieniek B, Berlin; Bihari A-S, Köln; Brust J, Mannheim; Buhk T, Hamburg; Busch HW, Münster; Carganico A, Berlin; Carls H, Düsseldorf; Christensen S, Münster; Claus J, Berlin; Cordes C, Berlin; Fätkenheuer G, Köln; Fenske S, Hamburg; Fischer K, Berlin; Ghosh I, Berlin; Gläßel F, München; Glaunsinger T, Berlin; Gute P, Frankfurt/Main; Hartl H, München; Herrmann S, Köln; Hintsche B, Berlin; Holm S, Hannover; Hower M, Dortmund; Isernhagen K, Köln; Jägel-Guedes E, München; Jäger H, München; Jessen A, Berlin; Karwat M, München; Klauke S, Frankfurt/Main; Klinker H, Würzburg; Knecht G, Frankfurt/Main; Knechten H, Aachen; Köppe S, Berlin; Köthemann W, Köln; Kreckel P, Berlin; Lichtenstein Z, München; Locher L, Frankfurt/Main; Lutz T, Frankfurt/Main; Markus R, Frank-furt/Oder; Mauss S, Düsseldorf; Mayr C, Berlin; Meyer A, Hamburg; Miasnikov V, Düsseldorf; Moll A, Berlin; Mösch M, Frankfurt; Mosthaf FA, Karlsruhe; Müller M, Stuttgart; Mutz A, Osnabrück; Pfeil B, Leipzig; Prziwara D, Berlin; Rausch M, Berlin; Reith M, Düsseldorf; Römer K, Köln; Scheidegger C, München; Schewe KK, Hamburg; Schleehauf D, Berlin; Schlote F, Berlin; Schmidt, Hannover; Schnaitmann E, Stuttgart; Scholten S, Köln; Schuster D, Mannheim; Seidel T, Jena; Starke W, Wiesbaden; Staszewski S, Frankfurt; Stellbrink HJ, Hamburg; Stephan C, Frankfurt; Stoehr A, Hamburg; Strehlow A, Düsseldorf; Stücker W, Köln; Träder C, Berlin; Ulmer A, Stuttgart; Van Lunzen J, Hamburg; Volkert R, München; Waizmann M, Leipzig; Wünsche T, Berlin. The study was supported by Roche AG.
- Calza L, Manfredi R, Chiodo F: Dyslipidaemia associated with antiretroviral therapy in HIV-infected patients. J Antimicrob Chemother 2004,53(1):10–14. ReviewPubMedView ArticleGoogle Scholar
- Koppel K, Bratt G, Eriksson M, Sandström E: Serum lipid levels associated with increased risk for cardiovascular disease is associated with highly active antiretroviral therapy (HAART) in HIV-1 infection. Int J STD AIDS 2000,11(7):451–455. 10.1258/0956462001916236PubMedView ArticleGoogle Scholar
- DAD Study Group, Friis-Møller N, Reiss P, Sabin CA, Weber R, Monforte A, El-Sadr W, Thiébaut R, De Wit S, Kirk O, Fontas E, Law MG, Phillips A, Lundgren JD: Class of antiretroviral drugs and the risk of myocardial infarction. N Engl J Med 2007,356(17):1723–1735.View ArticleGoogle Scholar
- Walmsley S, Cheung AM, Fantus G, Gough K, Smaill F, Azad , Diong C, Raboud J: A prospective study of body fat redistribution, lipid, and glucose parameters in HIV-infected patients initiating combination antiretroviral therapy. HIV Clin Trials 2008,9(5):314–323. 10.1310/hct0905-314PubMedView ArticleGoogle Scholar
- Palacios R, Merchante N, Macias J, González M, Castillo J, Ruiz J, Márquez M, Gómez-Mateos J, Pineda JA, Santos J: Incidence of and risk factors for insulin resistance in treatment-naïve HIV-infected patients 48 weeks after starting highly active antiretroviral therapy. Antivir Ther 2006,11(4):529–535.PubMedGoogle Scholar
- Oldfield V, Plosker GL: Lopinavir/ritonavir: a review of its use in the management of HIV infection. Drugs 2006,66(9):1275–1299. 10.2165/00003495-200666090-00012PubMedView ArticleGoogle Scholar
- Goldsmith D, Perry C: Atazanavir. Drugs 2003,63(16):1679–1693. 10.2165/00003495-200363160-00003PubMedView ArticleGoogle Scholar
- Dragsted UB, Gerstoft J, Pedersen C, Peters B, Duran A, Obel N, Castagna A, Cahn P, Clumeck N, Bruun JN, Benetucci J, Hill A, Cassetti I, Vernazza P, Youle M, Fox Z, Lundgren JD, MaxCmin1 Trial Group: Randomized trial to evaluate indinavir/ritonavir versus saquinavir/ritonavir in human immunodeficiency virus type 1-infected patients: the MaxCmin1 Trial. J Infect Dis 2003,188(5):635–642. 10.1086/377288PubMedView ArticleGoogle Scholar
- Dragsted UB, Gerstoft J, Youle M, Fox Z, Losso M, Benetucci J, Jayaweera DT, Rieger A, Bruun JN, Castagna A, Gazzard B, Walmsley S, Hill A, Lundgren JD, MaxCmin2 Trial Group: A randomized trial to evaluate lopinavir/ritonavir versus saquinavir/ritonavir in HIV-1infected patients: the MaxCmin2 trial. Antivir Ther 2005,10(6):735–743.PubMedGoogle Scholar
- Ananworanich J, Gayet-Ageron A, Ruxrungtham K, Chetchotisakd P, Prasithsirikul W, Kiertiburanakul S, Munsakul W, Raksakulkarn P, Tansuphasawadikul S, Le-Braz M, Jupimai T, Ubolyam S, Schutz M, Hirschel B, Staccato Thailand Study Group: Long-term efficacy and safety of first-line therapy with once-daily saquinavir/ritonavir. Antivir Ther 2008,13(3):375–380.PubMedGoogle Scholar
- Walmsley S, Avihingsanon A, Slim J, Ward DJ, Ruxrungtham K, Brunetta J, Bredeek UF, Jayaweera D, Guittari CJ, Larson P, Schutz M, Raffi F: Gemini: A non-inferiority study of saquinavir/ritonavir versus lopinavir/ritonavir as initial HIV-1 therapy in adults. J Acquir Immune Defic Syndr 2009,50(4):367–374. 10.1097/QAI.0b013e318198a815PubMedView ArticleGoogle Scholar
- German and Austrian guidelines for the diagnosis and therapy of HIV-infection 2008.
- Hammer SM, Eron JJ Jr, Reiss P, Schooley RT, Thompson MA, Walmsley S, Cahn P, Fischl MA, Gatell JM, Hirsch MS, Jacobsen DM, Montaner JS, Richman DD, Yeni PG, Volberding PA, International AIDS Society-USA: Antiretroviral treatment of adult HIV infection: 2008 recommendations of the International AIDS Soci-ety-USA panel. JAMA 2008,300(5):555–570. 10.1001/jama.300.5.555PubMedView ArticleGoogle Scholar
- European AIDS Clinical Society Guidelines 2008. [http://www.eacs.eu/guide/index.htm]
- Salzberger B, Marcus U, Vielhaber B, Arasteh K, Gölz J, Brockmeyer NH, Rockstroh J: German-Austrian recommendations for the antiretroviral therapy of HIV-infection (status May 2004). Eur J Med Res 2004,9(11):491–504.PubMedGoogle Scholar
- Invirase ® Summary of product characteristics [http://emc.medicines.org.uk/document.aspx?documentId=16123]
- Bittner B, Riek M, Holmes B, Grange S: Saquinavir 500 mg film-coated tablets demonstrate bioequivalence to saquinavir 200 mg hard capsules when boosted with twice-daily ritonavir in healthy volunteers. Antivir Ther 2005,10(7):803–810.PubMedGoogle Scholar
- Valer L, De Mendoza C, De Requena DG, Labarga P, García-Henarejos A, Barreiro P, Guerrero F, Vergara A, Soriano V, Fortogene Spanish Study Group: Impact of HIV genotyping and drug levels on the response to salvage therapy with saquinavir/ritonavir. AIDS 2002,16(14):1964–1966. 10.1097/00002030-200209270-00016PubMedView ArticleGoogle Scholar
- de Mendoza C, Valer L, Ribera E, Barreiro P, Martín-Carbonero L, Ramirez G, Soriano V: Performance of six different ritonavir-boosted protease inhibitor-based regimens in heavily antiretroviral-experienced HIV-infected patients. HIV Clin Trials 2006,7(4):163–171. 10.1310/hct0704-163PubMedView ArticleGoogle Scholar