Figure 3
From: Development of a novel monoclonal antibody to B7-H4: characterization and biological activity
IP and western blotting analysis with 3E8 mAb. - A: Western blotting was carried out for B7-H4 from lysates of 3T3-mB7-H4 or 3T3 cells using 3E8 mAb, followed by goat anti-mouse IgM antibody coupled with HRP. B: Western blotting was carried out for B7-H4 from lysates of 3T3-mB7-H4 or 3T3 cells using goat anti-B7-H4 polyclonal antibody, followed by rabbit anti-goat IgG antibody coupled with HRP. 3E8 mAb (2 μ/sample). Western blotting was carried out for B7-H4 using goat anti-B7-H4 polyclonal antibody, followed by rabbit anti-goat IgG antibody coupled with HRP. Normal mouse IgM was used as an antibody control. Lane 1: lysate from normal 3T3 cells; Lane 2: lysate from 3T3-mB7-H4 cells; Lane 3: B7-H4 unrelated antibody control.