Volume 19 Supplement 1

Proceedings of the 1st International Conference of Collaborative Research Center 974: Liver Damage and Regeneration

Open Access

Enforced viral replication, a mechanism for immune activation

  • Karl S Lang1, 2Email author,
  • Nadine Honke1, 2,
  • Namir Shaabani1, 2,
  • Dieter Häussinger2 and
  • Philipp A Lang2
European Journal of Medical Research201419(Suppl 1):S26

DOI: 10.1186/2047-783X-19-S1-S26

Published: 19 June 2014

Since many decades, it is known that viral infection can lead to a strong immune activation. This partially explains the strong association of viral infection with autoimmunity, immunopathology during chronic virus infections and complications during transplantation. One explanation for the strong immune stimulatory effects of viruses is the expression of specific patterns (e.g. viral RNA). Pathogen-specific patterns can be recognized by endogenous receptors (e.g.: Toll -like receptors), which are known to induce a strong innate immune activation. Whether further immunological mechanisms contribute to virus-specific immune activation is current topic of research.

In the recently published work [1, 2], we identified the mechanism of enforced viral replication. We found that specialized cells in the spleen and lymph nodes (CD169+ macrophages and conventional dendritic cells) selectively allowed replication of viruses. This enforced viral replication led to an exorbitant propagation of viral antigens and viral RNA. Viral antigen led to massive activation of the adaptive immune system, whilst viral RNA activated innate immunity. Therefore the mechanism of enforced viral replication partially explains the association of strong immune activation with virus infection.

First, we analyzed the role of enforced viral replication during infection with cytopathic vesicular stomatitis virus (VSV). We demonstrated that CD169+ macrophages express the protein UBP43 (gene name: Usp18). UBP43 is a cellular interferon type I (IFN-I) inhibitor. Therefore, due to expression of UBP43, IFN-I could not induce an antiviral status in CD169+ macrophages. Thus VSV, once it infected CD169+ macrophages, showed strong replication in this particular cell type. Other types of macrophages (Kupffer cells and red pulp macrophages) did not show VSV replication after infection. The enhanced replication of VSV in CD169+ macrophages induced a strong immune activation. Genetic depletion of Usp18 or depletion of CD169+ macrophages limited enforced viral replication and resulted in a reduced activation of the adaptive and innate immune response. As a consequence virus could not be controlled resulting in fast death after infection. Together our data show that replication of VSV in specific splenic niches is essential for innate and adaptive immune activation and for surviving an infection with cytopathic VSV.

To investigate the contribution of enforced viral replication to autoimmune disorders we tested its role in the induction of autoimmune diabetes using the RIP-GP mouse model. RIP-GP mice express the glycoprotein of the lymphocytic choriomeningitis virus (LCMV) under the rat insulin promoter. LCMV-specific CD8+ T cells, which usually develop during LCMV infection, show cross-reactivity to pancreatic beta cells of RIP-GP mice. Therefore RIP-GP mice develop diabetes during infection with LCMV. We found that induction of LCMV-specific CD8+ T cells as well as LCMV induced innate immune response depended on enforced viral replication. Genetic knockdown of Usp18, depletion of CD169+ macrophages and conventional dendritic cells or pharmacological inhibition of viral life cycle, blunted viral replication in the spleen and limited innate and adaptive immune activation against LCMV. Thereby the development of autoimmune diabetes was prevented.

In summary, professional innate immune cells purposely allow virus replication due to over-expression of UBP43. UBP43 expression and accelerated virus replication was essential to activate the antiviral innate and adaptive immune response. At the one hand this mechanism is absolutely required to protect from fatal virus infection, at the other hand it also strongly contributes to induction of virus induced autoimmune diabetes. In the future, it will be investigated in which other diseases this mechanism could play a role, and whether it may also have clinical implications.

Authors’ Affiliations

(1)
Institute of Immunology, Medical Faculty, University of Duisburg-Essen
(2)
Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich Heine University

References

  1. Honke N, Shaabani N, Zhang DE, Iliakis G, Xu HC, Häussinger D, Recher M, Löhning M, Lang PA, Lang KS: Usp18 driven enforced viral replication in dendritic cells contributes to break of immunological tolerance in autoimmune diabetes. PLoS Pathog 2013, 9: e1003650. 10.1371/journal.ppat.1003650PubMed CentralPubMedView ArticleGoogle Scholar
  2. Honke N, Shaabani N, Cadeddu G, Sorg UR, Zhang DE, Trilling M, Klingel K, Sauter M, Kandolf R, Gailus N, van Rooijen N, Burkart C, Baldus SE, Grusdat M, Löhning M, Hengel H, Pfeffer K, Tanaka M, Häussinger D, Recher M, Lang PA, Lang KS: Enforced viral replication activates adaptive immunity and is essential for the control of a cytopathic virus. Nat Immunol 2012, 13: 51–7.View ArticleGoogle Scholar

Copyright

© Lang et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Advertisement