Figure 4

mRNA and protein expression of germ-cell markers STELLA, VASA and DAZL in human umbilical cord mesenchymal stem cell–Sertoli cell co-cultures. Total (A) RNA and (B) protein were prepared from human umbilical cord mesenchymal stem cells (HUMSCs) cultured alone or co-cultured with Sertoli cells (SCs) for different durations and used for RT-PCR and Western blot analyses. Water was used as a negative control, and β-actin (ACTIN) served as a loading control.