Fig. 2

TH suppresses the migration and invasion of HCC cells. A Western Blotting was utilized to detect the overexpression of Flag-TH in HCC cell lines, including Huh7, PVTT, MHCC97H, HepG2, and QGY7701. B, C Transwell invasion assays (B, C) was conducted to measure the impact of TH overexpression on the invasion ability of HCC cells (B). These invasion cells were counted and analyzed (C). **P < 0.01; ****P < 0.0001. D, E Impact of TH overexpression on the migration of HCC cells was evaluated employing a wound-healing experiment (D). The relative healing areas were quantified and analyzed (E) ***P < 0.001; ****P < 0.0001. F Western blotting was conducted to measure the interfere impact of shRNA on the expression of TH protein in HCC cells. G, H Transwell invasion assays were applied to investigate the impact of suppressing TH on the invasion ability of Huh7, PVTT, as well as MHCC97H cell lines (G). The number of invasive cells was then counted and analyzed (H). The presence of scale bars was highlighted. ***P < 0.001; ****P < 0.0001