Fig. 3
From: Tyrosine hydroxylase inhibits HCC progression by downregulating TGFβ/Smad signaling
TH suppresses the proliferation and anchor-independent growth of HCC cells. A, B Impact of TH overexpression on the anchor-independent growth ability of HCC cells (HepG2, QGY7701, and MHCC97H) was evaluated by applying the soft-agar assays (A). The colony number was counted and analyzed (B). ***P < 0.001. C, D Soft-agar assays was conducted to examine the impact of TH knockdown on the anchor-independent growth ability of Huh7 cell lines (C). The number of colonies were counted and analyzed (D). The statistical significance levels are as follows: ****P < 0.0001. E Proliferation ability of HCC cells with TH overexpression was measured using the CCK8 assays. The presence of scale bars was highlighted. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. F The CCK8 assay was conducted to test the proliferation rate of HCC cells with TH knock-down. **P < 0.01; ***P < 0.001; ****P < 0.0001