Fig. 4
From: Tyrosine hydroxylase inhibits HCC progression by downregulating TGFβ/Smad signaling
TH interacts with Smad2 in a TGFβ1-dependent manner. A Co-immunoprecipitation was conducted to identify the interaction involving exogenously expressed Flag-TH and HA–Smad2 in HEK293T cells. B Endogenously interaction between TH and Smad2 in Huh7 cells was detected by performing Co-immunoprecipitation. C GST pull-down assay was conducted to identify the directly binding of the GST–Smad2 fusion protein and the exogenously expressed Flag-TH. D Co-immunoprecipitation was applied to measuring the impact of TGFβ1 treatment on the interaction between exogenously expressed Flag-TH and endogenously expressed Smad2 in Huh7 cell lines