Fig. 5
From: Tyrosine hydroxylase inhibits HCC progression by downregulating TGFβ/Smad signaling
TH suppresses the activation of TGFβ/Smad signaling pathway. A Phosphorylation level of Smad2 (S465/467) in Huh7 and PVTT cells treated with TGFβ1 was measured by utilizing Western blotting to evaluate the impact of TH overexpression. B Phosphorylation level of Smad2 (S465/467) in Huh7 cells with TH knockdown treated with TGFβ1 was evaluated by western blotting. C qPCR was conducted to identify the impact of TH overexpressed in Huh7 and PVTT cell lines on the translational induction of CTGF mRNA in response to TGFβ1 treatment. *P < 0.05; ***P < 0.001. D Western blotting was conducted to evaluate the impact of THWT, THS19A and THS40A on the phosphorylation of Smad2 (S465/467) in Huh7 and PVTT cells. E qPCR was applied to identify the impact of THWT, THS19A and TH.S40A overexpression on the mRNA levels of CTGF after treating Huh7 and PVTT cell lines with TGF-β1. *P < 0.05; **P < 0.01; ***P < 0.001