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Fig. 2 | European Journal of Medical Research

Fig. 2

From: Concentration strategies for spiked and naturally present biomarkers in non-invasively collected first-void urine

Fig. 2

Boxplots representing the amount of A GAPDH; B PhHV-1; C HPV16; and D PsV (EGFP) DNA in the respective samples. Results are presented as nanograms (ng), arbitrary DNA copies, and DNA copies. The spiked—No PEG arm did not undergo PEG treatment, whereas the other arms were incubated with 10% (w/v) PEG6000 or 8% (w/v) PEG20000 for approximately 16 h at 4 °C. All the samples were centrifuged at 3000×g at 4 °C for 1 h and the low-speed centrifugation (LSC) samples were subjected to low-speed centrifugation without PEG addition at 1000×g for 5 min before subsequent PEG precipitation. Significant differences between the pellet and supernatant for each arm are presented in the figure

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