Table 1 Overview of DNA and UMOD quantities (median, IQR) in different fractions of various arms
GAPDH (ng) | PhHV-1 (arbitrary copies) | HPV16 (DNA copies) | PsV (EGFP) (arbitrary copies) | UMOD (ng) | |
|---|---|---|---|---|---|
No PEG | |||||
Baseline | 311 (76–807) | 1709 (1380–2120) | 1330 (1110–1635) | 189,500 (125,000–404500) | 7.62 (4.71–10.84) |
Low-speed centrifugation | |||||
Pellet | 193 (41–388) | 109 (52–638) | 78 (58–255) | 74,000 (48,138–107,125) | |
Supernatant | 23 (12–55) | 1131 (961–1473) | 979 (861–1223) | 50,730 (32,728–130,388) | |
Unspiked—no PEG | |||||
Pellet | 193 (51–756) | 0 (0–0) | 0 (0–0) | 0 (0–0) | |
Supernatant | 14 (12–42) | 0 (0–0) | 0 (0–0) | 0 (0–0) | |
Spiked—no PEG | |||||
Pellet | 314 (80–786) | 277 (66–984) | 162 (98–1068) | 177,500 (142,250–350000) | 13.62 (8.19–17.98) |
Supernatant | 11 (7–35) | 768 (674–1169) | 766 (408–1102) | 20,093 (11,329–29,403) | 2.01 (1.80–2.64) |
PEG | |||||
PEG6000 | |||||
Pellet | 384 (104–1244) | 928 (709- 2064) | 1415 (133–1584) | 266,750 (211,750–697125) | 12.69 (4.36–21.33) |
Supernatant | 1 (0–3) | 118 (18–294) | 47 (10–79) | 2106 (1331–2642) | 2.36 (1.49–3.35) |
PEG20000 | |||||
Pellet | 486 (154–1323) | 1188 (617–2130) | 1290 (1103–1468) | 197,500 (180,375–224375) | 5.43 (3.49–19.35) |
Supernatant | 0 (0–3) | 346 (0–768) | 65 (17–86) | 2447 (1149–5572) | 2.94 (1.98–4.00) |
Low-speed centrifugation + PEG6000 | |||||
Pellet | 20 (11–43) | 630 (226–943) | 643 (549–1011) | 67,500 (51,063–90750) | 0.3 (0.19–0.36) |
Supernatant | 2 (0–3) | 224 (129–522) | 116 (59–185) | 2485 (841–4870) | 1.80 (1.30–2.09) |
Low-speed centrifugation + PEG20000 | |||||
Pellet | 15 (12–42) | 620 (386–775) | 615 (410–771) | 67,100 (21,750–106500) | 0.23 (0.16–0.31) |
Supernatant | 3 (1–3) | 226 (141–366) | 211 (115–246) | 9380 (6387–12,034) | 2.48 (1.21–3.14) |