Fig. 6

TR4 promotes pyroptosis during urosepsis. A Propidium iodide (PI; red indicates dead cells) and Hoechst (blue indicates all nuclei) double staining were used to observe the morphology of pyroptotic cell, and the numbers of pyroptotic cells were compared; *p < 0.05, **p < 0.01, ***p < 0.001. B Comparison of the IL-1β concentration in the culture supernatant of control and TR4-overexpressing macrophages; *p < 0.05, **p < 0.01, ***p < 0.001. C Comparison of 24-h pyroptotic cell numbers in control and TR4-overexpressing macrophages