Fig. 7

TR4 regulates the expression of GSDMD. A The expression of GSDMD mRNA was changed when TR4 was overexpressed or knocked down, *p < 0.05, **p < 0.01, ***p < 0.001. B Changes in the protein level of GSDMD when TR4 was overexpressed or knocked down. The quantification of TR4 and GSDMD expression based on band density is shown in the right panel. C Correlation between TR4 and GSDMD expression in blood cells. D The upper picture shows the motif structure of TR4 predicted by the website, and the lower picture shows the promoter region of GSDMD to which TR4 may bind, which was predicted by the hTFtarget website. E ChIP experiments verified that TR4 binds to the GSDMD promoter region (chr8:143553465–143,553867); **p < 0.01