Table 2 The primer sequences of studied genes
From: CD133 significance in glioblastoma development: in silico and in vitro study
Gene | Forward primer | Reverse primer |
|---|---|---|
MMP16 | CCCACACCGCTCTATTCCTCC | CCCTGTTGTTTCTCACTCGCC |
SOX2 | AAAACAGCCCGGACCGCGTC | CTCGTCGATGAACGGCCGCT |
RAF1 | AGTCACAGCGAATCAGCCTC | GCCTAATTTTGTTTTTCTCCTGGG |
MAP2K1 | CAATGGCGGTGTGGTGTTC | GATTGCGGGTTTGATCTCCAG |
MAPK3 | CGCTTCCGCCATGAGAATGT | GGTCAGTCTCCATCAGGTCC |
PIK3CA | GAAGCACCTGAATAGGCAAGTCG | GAGCATCCATGAAATCTGGTCGC |
AKT3 | AGAACGACCAAAGCCAAACACA | AGTCTGTCTGCTACAGCCTGG |
mTOR | AGATGCTTGGAACCGGACCTG | CCAAGATGCCACCTTTCCTCTC |
CDK4 | CCATCAGCACAGTTCGTGAGGT | TCAGTTCGGGATGTGGCACAGA |
BCL2 | ACTGGCTCTGTCTGAGTAAG | CCTGATGCTCTGGGTAAC |
CD133 | GCTTTGCAATCTCCCTGTTG | TTGATCCGGGTTCTTACCTG |
18s | ACCCGTTGAACCCCATTCGTGA | GCCTCACTAAACCATCCAATCGG |