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Table 2 Thermal cycler parameters for quantitative real-time PCR

From: Photodynamic therapy upregulates expression of HIF-1α and PD-L1 in related pathways and its clinical relevance in non-small-cell lung cancer

Initial denaturation

 50 °C

2 min

 95 °C

2 min

Amplification cycle (40 cycles)

 95 °C

15 s

 60 °C

15 s

 72 °C

60 s

Final extension

 60 °C

10 min

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European Journal of Medical Research

ISSN: 2047-783X

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