Fig. 8

Silencing key gene PFKFB3 suppressed papillary renal cell carcinoma proliferation, migration and sunitinib resistance. A qRT-PCR to confirm the performance of siRNA targeting PFKFB3. B CCK-8 assay results indicated that PFKFB3 knockdown decreased cell proliferation. C Colony-formation efficiency of knockdown PFKFB3. D Transwell migration assay of knockdown PFKFB3 and control group. E Glucose uptake, lactate detection, and ATP detection assays of knockdown PFKFB3. F Glucose uptake, lactate detection, and ATP detection assays of ACHN cells at 1μM PFK-015. G Inhibition rate of ACHN siNC or siPFKFB3 cells treated with sunitinib. H The growth of ACHN cells after the treatment with sunitinib and/or PFK-015 for 10 days was determined using colony formation assay. I–L Synergy score plot based on ZIP (I); Loewe (J); Bliss (K) and HSA (L) models