Fig. 2

Knockdown of circSOD2 attenuates PA-induced hepatocyte pyroptosis. transfection of si-circSOD2#1 and si-circSOD2#2 into PA-treated AML12 cells. A Detection of circSOD2 expression by RT-qPCR; B pyroptotic cells assessed via flow cytometry; C expression of pyroptosis-related proteins IL-1β, IL-18, GSDMD, cleaved caspase-1, and phosphorylated p65 analyzed by Western blot; D levels of TNF-α, IL-1β, IL-6, SOD, and MDA determined by ELISA or conventional assay kits; E LDH release measured using assay kits; F MMP assessed by JC-1 staining (100 × , 20 μm); data are presented as mean  ± SD (n = 3).*P < 0.05