Fig. 4

Overexpression of miR-532-3p alleviates PA-induced hepatocyte pyroptosis. Introduction of miR-532-3p mimic into PA-treated AML12 cells. A miR-532-3p expression monitored by RT-qPCR;B pyroptotic cells quantified by flow cytometry; C Western blot analysis of pyroptosis-associated proteins IL-1β, IL-18, pro IL-1β, pro IL-18, cleaved GSDMD, cleaved caspase-1, and phosphorylated p65; D concentrations of TNF-α, IL-1β, IL-6, SOD, and MDA measured by ELISA or standard assay kits; E LDH release evaluated using assay kits; F MMP assessed by JC-1 staining (100 × , 20 μm); data are represented as mean ± SD (n = 3). *P < 0.05