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Fig. 5 | European Journal of Medical Research

Fig. 5

From: Competitive adsorption of microRNA-532-3p by circular RNA SOD2 activates Thioredoxin Interacting Protein/NLR family pyrin domain containing 3 pathway and promotes pyroptosis of non-alcoholic fatty hepatocytes

Fig. 5

The inhibitory effect of circSOD2 knockdown on AML cell pyroptosis is reversed by knockdown of miR-532-3p. Transfection of miR-532-3p inhibitor into circSOD2-knockdown AML12 cells. A Measurement of miR-532-3p expression by RT-qPCR; B detection of pyroptotic cells by flow cytometry; C analysis of pyroptosis-related proteins IL-1β, IL-18, GSDMD, cleaved caspase-1, and phosphorylated p65 by Western blot; D levels of TNF-α, IL-1β, IL-6, SOD, and MDA determined by ELISA or conventional assay kits; E Assessment of LDH release with assay kits; F MMP evaluated by JC-1 staining (100 × , 20 μm); data are presented as mean  ± SD (n = 3) *P < 0.05.

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European Journal of Medical Research

ISSN: 2047-783X

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