Fig. 7

Knockdown of TXNIP prevents PA-induced hepatocyte pyroptosis by downregulating NLRP3. Transfection of si-TXNIP#1 and si-TXNIP#2 into PA-treated AML12 cells. A TXNIP and NLRP3 expression analyzed by Western blot; B pyroptotic cells quantified by flow cytometry; C expression of pyroptosis-associated proteins IL-1β, IL-18, GSDMD, cleaved caspase-1, and phosphorylated p65 assessed by Western blot; D concentrations of TNF-α, IL-1β, IL-6, SOD, and MDA measured by ELISA or standard assay kits; E LDH release evaluated using assay kits; F MMP assessed by JC-1 staining (100 × , 20 μm); data are presented as mean ± SD (n = 3). *P  < 0.05