Fig. 8

circSOD2 modulates PA-induced hepatocyte pyroptosis through the miR-532-3p/TXNIP axis. Co-transfection of pcDNA 3.1-circSOD2 and si-TXNIP#1 into PA-treated AML12 cells. A Expression of circSOD2 and miR-532-3p assessed by RT-qPCR; B TXNIP and NLRP3 expression evaluated by Western blot; C quantification of pyroptotic cells via flow cytometry; D analysis of pyroptosis-related proteins IL-1β, IL-18, GSDMD, cleaved caspase-1, and phosphorylated p65 by Western blot; E levels of TNF-α, IL-1β, IL-6, SOD, and MDA determined by ELISA or conventional assay kits; F LDH release measured using assay kits; G MMP assessed by JC-1 staining (100 × , 20 μm); data are represented as mean  ± SD (n = 3) *P < 0.05.