Fig. 2

Role of Jian Gan powder (JGP) in BCG + LPS-induced immunological liver injury (ILI) in BALB/c mice. A Experimental schedule. B ELISA of the serum levels of interferon-gamma (IFN-γ), interleukin (IL)-6, IL-10, and IL-22. C Western blotting analysis of STAT3 and p-STAT3 protein levels in the liver of experimental and control groups (five mice per group). D Representative images of proliferating cell nuclear antigen (PCNA) staining (× 200) and the percentage of PCNA-positive cells (arrowhead). (A normal group; B model group [induced for experimental ILI]; C positive control group; D JGP-L group; E: JGP-M group; F: JGP-H group). Bars: 100 μm. Data were analyzed using one-way analysis of variance and were presented as mean ± SEM. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001