(A) Experimental design for Saireito therapy of nifedipine-induced gingival overgrowth in rats. The numbers in parentheses represent the number of rats used for each of the experiments. (B) Measurements of incisor space and gingiva width. The space between the tips of the mandibular incisors and the width of buccal gingival together with the maxillary left first molar were measured as the incisor space and the gingiva width, respectively. (C) Morphometric analysis of gingival overgrowth. The area of an orange-colored part (connective tissue) surrounded by a dotted line was measured.