Shows a cross section of the ear of the hairless mouse. On the left in hematoxylin-eosin, on the right in TUNEL staining 72 h post trauma. The picture on the left shows a cluster of proteins (eosinophilic (a)) in the area of necrosis/no perfusion (b) followed by an area of granulocytes (purple (c)), marking off the vital (e) from the avital (b) tissue; (d) marks the border between vital and avital tissue. TUNEL staining was used to find the exact border of necrotic/apoptotic and vital tissue. The vital cells stain blue (f) and the necrotic/apoptotic cells red (g).