A-2C, mRNA expression of LIGHT in immune cells. T cells were stimulated with PMA and ionomycin for 2, 4 or 16 hours. PMA differentiated THP-1 cells, monocyte-derived macrophages were further stimulated with TNF-α for 2, 4 or 16 hours. Expression of LIGHT was assessed at different time points by RT-PCR. Maximum of LIGHT expression in T cells was observed after 2 hours after stimulation (Figure 2A). *P < 0.05 vs unstimulated cells. In differentiated THP-1 cells and monocyte-derived macrophages maximum LIGHT expression peaked after 4 hours at the mRNA-level (Figure 2B and 2C). *P < 0.05 vs monocytes and THP-1 cells. These data correspond to one representative experiment from three performed.