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Figure 1 | European Journal of Medical Research

Figure 1

From: Airway surface liquid contains endogenous DNase activity which can be activated by exogenous magnesium

Figure 1

Digestion of restricted plasmid DNA (pEGFP-N1, generated by restriction with EcoRI and NotI) by BAL samples or DNase I (0.05 U/μg DNA). M: Molecular marker; P1: Restriction fragments of plasmid DNA and DNase 0.05 U/μg DNA; P2: Restriction fragments of plasmid DNA and DNase 0.05 U/μg in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; BAL1 first lane: Restriction fragments of plasmid DNA and 130 μl BAL 1; second lane: Restriction fragments of plasmid DNA and 130 μl BAL 1in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; BAL 2 first lane: Restriction fragments of plasmid DNA and 130 μl BAL 2; second lane: Restriction fragments of plasmid DNA and 130 μl BAL 2 in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; BAL 3 first lane: Restriction fragments of plasmid DNA and 130 μl BAL 3; second lane: Restriction fragments of plasmid DNA and 130 μl BAL 3 in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; BAL 4 first lane: Restriction fragments of plasmid DNA and 130 μl BAL 4; second lane: Restriction fragments of plasmid DNA and 130 μl BAL 4 in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2.

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