Table 1 Sequences of primers as used for PCR.
From: Angiotensin-converting enzyme I/D polymorphism in chronic obstructive pulmonary disease
Gene | Polymorphism | Primers | MgCl2 |
|---|---|---|---|
ACE | II/ID/DD | F: 5'-CTGGAGACCACTCCCATCCTTTCT-3' R: 5'-GATGTGGCCATCACATTCGTCAGAT-3' | 2 mM |
control PCR for detection of I, if DD in first PCR | F:5'-TGGGACCACAGCGCCCGCCACTAC-3' R:5'-TCGCCAGCCCTCCCATGCCCATAA-3' | 2, 5 mM |