Skip to main content

Table 1 PCR conditions for each gene.

From: Influence of proinflammatory cytokine gene polymorphism on childhood obesity

PCR stage IL-6 TNF IL-1
Initial denaturation 94°C
10 min
94°C
3 min
94°C
4 min
Denaturation 94°C
1 min
94°C
1 min
94°C
30 s
Annealing 55°C
35 s
60°C
1 min
63°C
30 s
Elongation 72°C
1 min
72°C
1 min
72°C
30 s
Cycles number 35 36 35
Final elongation 72°C
10 min
72°C
5 min
72°C
10 min