Table 1 PCR conditions for each gene.

Initial denaturation

94°C

10 min

94°C

3 min

94°C

4 min

Denaturation

94°C

1 min

94°C

1 min

94°C

30 s

Annealing

55°C

35 s

60°C

1 min

63°C

30 s

Elongation

72°C

1 min

72°C

1 min

72°C

30 s

Cycles number

35

36

35

Final elongation

72°C

10 min

72°C

5 min

72°C

10 min