Figure 2
a) Methylation-specific PCR analysis of various cervical cancer cell lines and all of primary tumors and normal tissues. Bisulfite-treated DNA was used as a template for methylation-specific PCR by using previously described primers. M = primers specific to methylated template DNA, U = primers specific to unmethylated template DNA, T = tumor, N = normal b) RIZ1 mRNA expression in methylated and unmethylated cervical cancer tissues. c) Methylation status of SiHa cell line before and after treatment with the DNA methyltransferase-inhibiting agent, 5-aza-dC.