Figure 10bFrom: Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues Overlay of the densitogram curves before and after trypsin-incubation of the white muscle extract. Figure 10b shows 200 μg white muscle extracts before (green lane) and after (red lane) incubation with 65 μg trypsin (sees Material). The batches, incubated with trypsin had a final concentration of 20 mM Tris/HCl, 20 mM β-mercaptoethanol, pH 8.0. After incubation at 37°C for a given time (30 min) in a waterbath, the reaction were irreversible inhibited by an final concentration of 10% w/v TCA (final concentration 5% TCA, 20 min, 0°C).Back to article page