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Figure 9b | European Journal of Medical Research

Figure 9b

From: Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues

Figure 9b

Overlay of the densitogram curves before and after trypsin-incubation of the heart muscle extract. Figure 9b shows 200 μg heart muscle extracts before (green) and after (red) incubation with 65 μg trypsin (sees Material). The batches, incubated with trypsin had a final concentration of 20 mM Tris/HCl, 20 mM β-mercaptoethanol, pH 8.0. After incubation at 37°C for a given time (30 min) in a waterbath, the reaction were irreversible inhibited by an final concentration of 10% w/v TCA (final concentration 5% TCA, 20 min, 0°C).

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