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Table 1 Amino acid analysis of the first order ubiquityl-calmodulin conjugate

From: Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues

Amino Acid First order Ubiquityl-Calmodulin Conjugate
  Composition from sequence Composition found
   mean (mol/mol) stdev. (Mol/mol)
Asx 30 29.24 0.90
Glx 39 39.32 1.36
Ser 7 6.07 0.22
Gly 17 17.49 0.43
Thr 19 18.96 0.58
His 2 1.77 0.15
Ala 13 13.56 0.55
TM-lys 1 0.97 0.08
Arg 10 9.94 0.27
Tyr 3 3.16 0.12
Val 11 11.50 0.22
Met 10 9.88 0.23
Phe 10 10.69 0.33
Ile 15 15.56 1.12
Leu 18 18.34 0.73
Lys 14 13.19 1.25
  1. Isolated ubiquitin, calmodulin and monoubiquitylation products of calmodulin were hydrolyzed in vacuum for 24 respectively 48 hours in 6 N HCL, 0.1% (w/v) phenol at 110°C. Amino acid analysis (OPA method) was done on a Spherisorb O.D.S. II column (Fa. Grom, Herrenberg) as described in [1, 2]. The mean values were calculated from the data obtained after 24 and 48 hours of hydrolysis. Asx: asparagine and aspartic acid; Glx: glutamine and glutamic acid; TM-Lys: trimethyllysine.