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Table 1 Amino acid analysis of the first order ubiquityl-calmodulin conjugate

From: Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues

Amino Acid

First order Ubiquityl-Calmodulin Conjugate

 

Composition from sequence

Composition found

  

mean (mol/mol)

stdev. (Mol/mol)

Asx

30

29.24

0.90

Glx

39

39.32

1.36

Ser

7

6.07

0.22

Gly

17

17.49

0.43

Thr

19

18.96

0.58

His

2

1.77

0.15

Ala

13

13.56

0.55

TM-lys

1

0.97

0.08

Arg

10

9.94

0.27

Tyr

3

3.16

0.12

Val

11

11.50

0.22

Met

10

9.88

0.23

Phe

10

10.69

0.33

Ile

15

15.56

1.12

Leu

18

18.34

0.73

Lys

14

13.19

1.25

  1. Isolated ubiquitin, calmodulin and monoubiquitylation products of calmodulin were hydrolyzed in vacuum for 24 respectively 48 hours in 6 N HCL, 0.1% (w/v) phenol at 110°C. Amino acid analysis (OPA method) was done on a Spherisorb O.D.S. II column (Fa. Grom, Herrenberg) as described in [1, 2]. The mean values were calculated from the data obtained after 24 and 48 hours of hydrolysis. Asx: asparagine and aspartic acid; Glx: glutamine and glutamic acid; TM-Lys: trimethyllysine.