Skip to main content

Table 2 Aminoacid analysis of ubiquitin and calmodulin released from first-order ubiquitylcalmodulin conjugate

From: Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues

Amino Acid    UBIQUITIN     CALMODULIN   
   Control Released   Control Released
  Composition from Sequence Mean stdev. mean stdev. Composition from Sequence mean stdev. mean stdev.
  (mol/mol) (mol/mol) (mol/mol) (mol/mol) (mol/mol) (mol/mol) (mol/mol) (mol/mol) (mol/mol) (mol/mol)
Asx 7 7.0 0.35 7.2 0.26 23 22.7 1.85 23.0 0.96
Glx 12 12.4 0.95 12.7 0.86 27 28.1 1.75 26.8 3.09
Ser 3 2.6 0.12 2.8 0.17 4 3.8 0.22 4.3 0.36
Gly 6 6.7 0.22 6.1 0.52 11 11.0 0.96 10.6 0.72
Thr 7 6.5 0.32 6.4 0.13 12 11.9 0.76 11.2 0.49
His 1 0.9 0.18 0.8 0.06 1 0.9 0.15 1.3 0.44
Ala 2 2.1 0.12 2.1 0.12 11 11.6 0.36 11.2 0.76
TM-Lys 0 0.0 0.00 0.0 0.00 1 0.8 0.06 0.9 0.31
Arg 4 3.9 0.20 3.9 0.10 6 5.7 0.31 5.7 0.43
Tyr 1 0.9 0.14 1.0 0.04 2 2.1 0.08 2.0 0.20
Val 4 4.0 0.21 4.3 0.39 7 7.4 0.32 7.3 0.52
Met 1 nD nD 0.9 0.07 9 5.8 0.34 7.2 0.11
Phe 2 1.9 0.09 2.0 0.11 8 8.3 0.35 7.9 0.76
Ile 7 6.8 0.30 6.6 0.23 8 8.6 0.67 8.4 0.55
Leu 9 8.7 0.46 8.9 0.30 9 9.7 0.45 9.6 0.51
Lys 7 6.5 0.39 7.3 0.31 7 6.5 0.68 7.6 0.78
  1. The mean values were calculated from the data obtained after 24 and 48 hours of hydrolysis. For further details see legend to Table 1 and the text. ND: not determined