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Table 2 Aminoacid analysis of ubiquitin and calmodulin released from first-order ubiquitylcalmodulin conjugate

From: Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues

Amino Acid

  

UBIQUITIN

   

CALMODULIN

  
  

Control

Released

 

Control

Released

 

Composition from Sequence

Mean

stdev.

mean

stdev.

Composition from Sequence

mean

stdev.

mean

stdev.

 

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

(mol/mol)

Asx

7

7.0

0.35

7.2

0.26

23

22.7

1.85

23.0

0.96

Glx

12

12.4

0.95

12.7

0.86

27

28.1

1.75

26.8

3.09

Ser

3

2.6

0.12

2.8

0.17

4

3.8

0.22

4.3

0.36

Gly

6

6.7

0.22

6.1

0.52

11

11.0

0.96

10.6

0.72

Thr

7

6.5

0.32

6.4

0.13

12

11.9

0.76

11.2

0.49

His

1

0.9

0.18

0.8

0.06

1

0.9

0.15

1.3

0.44

Ala

2

2.1

0.12

2.1

0.12

11

11.6

0.36

11.2

0.76

TM-Lys

0

0.0

0.00

0.0

0.00

1

0.8

0.06

0.9

0.31

Arg

4

3.9

0.20

3.9

0.10

6

5.7

0.31

5.7

0.43

Tyr

1

0.9

0.14

1.0

0.04

2

2.1

0.08

2.0

0.20

Val

4

4.0

0.21

4.3

0.39

7

7.4

0.32

7.3

0.52

Met

1

nD

nD

0.9

0.07

9

5.8

0.34

7.2

0.11

Phe

2

1.9

0.09

2.0

0.11

8

8.3

0.35

7.9

0.76

Ile

7

6.8

0.30

6.6

0.23

8

8.6

0.67

8.4

0.55

Leu

9

8.7

0.46

8.9

0.30

9

9.7

0.45

9.6

0.51

Lys

7

6.5

0.39

7.3

0.31

7

6.5

0.68

7.6

0.78

  1. The mean values were calculated from the data obtained after 24 and 48 hours of hydrolysis. For further details see legend to Table 1 and the text. ND: not determined