Figure 2

Apoptotic function of target genes. Extent of apoptosis 96 h after treatment with CH11 in: A) populations of A375 (Melanoma), HS892-T (Kerathokanthoma), CLS-354 (Head and neck squamous cell carcinoma). B) Populations of ACHN (renal cell carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast adenocarcinoma). The cells were transiently transficted with either mock-vector (pTKO2) or with the different isolated antisense cDNA construct (encoding for anti-sense RNA molecules). After 48 h, the transected cells were exposed to CH11 for a further 48 h, followed by flow cytometric analysis using annexin V/PI. Data are the mean ± SD of three independent experiments performed separately.