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Table 3 Frequencies of methylated genes, CTC and combinations in association to clinico-pathological features.

From: Methylated APC and GSTP1 genes in serum DNA correlate with the presence of circulating blood tumor cells and are associated with a more aggressive and advanced breast cancer disease

    Number of Methylation PositivePatients    Number of CTC Positive Patients Methylation OR CTC pos.
Variables APC RASSF1A GSTP1 ESR1 Min-1
≥ 1 × pos.
(APC or RASSF1A, GSTP1)
Min-2
≥ 2 × pos.
(APC or RASSF1A, GSTP1)
CTC-2
≥ 1× pos CTC
(EPCAM or Muc1)
CTC-4
≥ 1× pos CTC
(EPCAM, Muc1 MGB1 or SPDEF)
COMBI
Min-2 or
CTC-2
ER          
   Positive 14/61 14/61 9/55 21/61 21/54 9/54 6/47 9/41 13/46
   Negative 5/11 3/11 3/11 3/11 7/11 3/11 3/10 3/9 5/10
PR          
   Positive 12/48 11/48 6/42 16/48 16/41 6/41 6/36 9/30 10/33
   Negative 8/26 8/26 6/25 12/26 13/25 7/25 3/23 3/20 9/24
Her2/neu          
   Negative 11/46 8/46 4/44 14/46 17/43 3/43 5/33 6/28 8/34
   Positive 7/20 7/20 7/17 12/20 9/17 7/17 4/18 4/16 8/17
    **p = 0.003 *p = 0.024   **p = 0.001    p = 0.088
CA15.3          
   Negative 9/52 12/52 7/50 20/52 20/49 6/49 5/50 7/43 10/48
   Positive 8/10 4/10 4/10 4/10 8/10 5/10 6/9 7/8 8/9
  **p = 0.000     *p = 0.024 **p = 0.005 **p = 0.000 **p = 0.000 **p = 0.000
Tumor size          
   T1+T2 16/61 11/61 6/57 21/61 22/56 6/56 8/49 11/41 12/48
   T3+T4 5/13 6/13 6/11 7/13 9/11 5/11 3/10 3/10 6/10
   *p = 0.029 **p = 0.000   **p = 0.010 **p = 0.004    *p = 0.030
LN State          
   Negative 7/32 8/32 3/32 11/32 13/31 4/31 2/28 5/26 6/27
   Positive 15/43 13/43 10/37 18/43 19/37 10/37 9/31 9/26 15/32
        *p = 0.031   *p = 0.049
M State          
   Negative 6/35 7/35 4/33 13/35 11/32 4/32 2/29 4/24 5/28
   Positive 14/25 11/25 5/21 9/25 14/21 9/21 7/19 8/18 14/21
  **p = 0.002 *p = 0.046    *p = 0.021 *p = 0.012 **p = 0.009 *p = 0.049 **p = 0.001
AJCC-lndex          
   0+ I 3/20 5/20 2/19 10/20 6/19 2/19 0/18 0/16 2/18
   II 2/22 0/22 1/20 6/22 2/19 0/19 2/15 4/14 2/13
   III 5/12 4/12 4/12 6/12 9/12 3/12 3/10 3/7 4/11
   IV 12/23 10/23 5/19 8/23 12/19 8/19 7/18 8/17 13/19
  **p = 0.001 *p = 0.031    *p = 0.022 **p = 0.003 **p = 0.002 **p = 0.003 **p = 0.001
Disease status          
   FD 13/64 13/64 10/59 26/64 23/58 9/58 7/49 9/44 13/48
   RD 10/19 8/19 3/15 5/19 11/15 5/15 5/13 6/11 9/14
  **p = 0.006     *p = 0.020   *p = 0.050 *p = 0.023 *p = 0.023
Group          
    Tumor 29% 25/85 26% 22/85 18% 14/76 38% 32/85 48% 36/75 20% 15/75 19% 12/63 27% 15/55 35% 22/62
   Control 2/22 5/22 1/16 5/22 6/16 2/16 2/14 2/14 8/30
  *p = 0.050         
  1. p < 0.05; ** p < 0.005 high significant