Table 4 Frequencies of methylated genes in association to circulating tumor cells.
Patients | Number of methylation positive Patients | ||||||
|---|---|---|---|---|---|---|---|
CTC Parameter: | APC | RASSF1A | GSTP1 | ESR1 | 1 × pos. (APC, RASSF1A, GSTP1) | 2 × pos. (APC, RASSF1 A, GSTP1) | |
EPCAM (Adnagen) | 13% 8/63 | ||||||
Negative | 11/54 | 12/54 | 17/54 | 6/53 | 21/52 | 7/52 | |
Positive | 6/8 | 4/8 | 5/7 | 5/8 | 6/7 | 4/7 | |
**p = 0.001 | **p = 0.001 | *p = 0.024 | **p = 0.005 | ||||
MUC1 (Adnagen) | 16% 10/63 | ||||||
Negative | 11/52 | 13/52 | 7/51 | 17/50 | 21/50 | 8/50 | |
Positive | 6/10 | 3/10 | 4/9 | 5/10 | 6/9 | 3/9 | |
*p = 0.012 | *p = 0.028 | ||||||
MGBI(TaqMan) | 9% 5/55 | ||||||
Negative | 13/49 | 12/49 | 9/48 | 20/49 | 21/47 | 9/47 | |
Positive | 2/5 | 2/5 | 0/4 | 1/5 | 1/4 | 1/4 | |
SPDEF(TaqMan) | 12% 7/58 | ||||||
Negative | 11/50 | 13/50 | 7/49 | 20/50 | 21/48 | 8/48 | |
Positive | 4/7 | 3/7 | 3/6 | 2/7 | 3/6 | 3/6 | |
*p = 0.048 | *p = 0.032 | ||||||
EPCAM or MUC1 | 19% 12/63 | ||||||
Negative | 10/50 | 12/50 | 6/49 | 17/50 | 20/48 | 7/48 | |
Positive | 7/12 | 4/12 | 5/11 | 5/12 | 7/11 | 4/11 | |
**p = 0.008 | *p = 0.010 | ||||||
≥ 1× pos (EPCAM, MUC1, MGB1, SPDEF) | 27% 15/55 | ||||||
Negative | 7/39 | 9/39 | 4/38 | 16/39 | 14/37 | 5/37 | |
Positive | 8/15 | 5/15 | 5/14 | 5/15 | 8/14 | 5/14 | |
**p = 0.009 | *p = 0.033 | ||||||
≥ 2× pos (EPCAM, MUC1, MGB1, SPDEF) | 18% 10/55 | ||||||
Negative | 9/44 | 10/44 | 4/43 | 16/44 | 16/44 | 6/42 | |
Positive | 6/10 | 4/10 | 5/9 | 5/10 | 6/9 | 4/9 | |
*p = 0.012 | *p = 0.001 | *p = 0.039 | |||||