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Table 4 Frequencies of methylated genes in association to circulating tumor cells.

From: Methylated APC and GSTP1 genes in serum DNA correlate with the presence of circulating blood tumor cells and are associated with a more aggressive and advanced breast cancer disease

  Patients Number of methylation positive Patients
CTC Parameter:   APC RASSF1A GSTP1 ESR1 1 × pos.
(APC, RASSF1A, GSTP1)
2 × pos.
(APC, RASSF1 A, GSTP1)
EPCAM (Adnagen) 13% 8/63       
   Negative   11/54 12/54 17/54 6/53 21/52 7/52
   Positive   6/8 4/8 5/7 5/8 6/7 4/7
   **p = 0.001   **p = 0.001   *p = 0.024 **p = 0.005
MUC1 (Adnagen) 16% 10/63       
   Negative   11/52 13/52 7/51 17/50 21/50 8/50
   Positive   6/10 3/10 4/9 5/10 6/9 3/9
   *p = 0.012   *p = 0.028    
MGBI(TaqMan) 9% 5/55       
   Negative   13/49 12/49 9/48 20/49 21/47 9/47
   Positive   2/5 2/5 0/4 1/5 1/4 1/4
SPDEF(TaqMan) 12% 7/58       
   Negative   11/50 13/50 7/49 20/50 21/48 8/48
   Positive   4/7 3/7 3/6 2/7 3/6 3/6
   *p = 0.048   *p = 0.032    
EPCAM or MUC1 19% 12/63       
   Negative   10/50 12/50 6/49 17/50 20/48 7/48
   Positive   7/12 4/12 5/11 5/12 7/11 4/11
   **p = 0.008   *p = 0.010    
≥ 1× pos (EPCAM, MUC1, MGB1, SPDEF) 27% 15/55       
   Negative   7/39 9/39 4/38 16/39 14/37 5/37
   Positive   8/15 5/15 5/14 5/15 8/14 5/14
   **p = 0.009   *p = 0.033    
≥ 2× pos (EPCAM, MUC1, MGB1, SPDEF) 18% 10/55       
   Negative   9/44 10/44 4/43 16/44 16/44 6/42
   Positive   6/10 4/10 5/9 5/10 6/9 4/9
   *p = 0.012   *p = 0.001    *p = 0.039
  1. * p < 0.05; ** p < 0.005 high significant