Detection of Plasmodium falciparum merozoite surface protein-1 (MSP-1)-specific CD4
+ T-cells in a healthy adult living in Nouna, Burkina Faso using the Fastlmmune short term lymphocyte activation assay. The columns starting from the left depict results obtained with highly purified IgG isotype control antibodies, or antibodies against interferon gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor-alpha (TNF-α). The upper row shows the unstimulated control sample (US), the middle row the positive control sample stimulated with Staphylococcal Enerotoxin B (SEB) and the lower row the sample stimulated with MSP-1. In the upper left quadrant of each dot plot the number of acquired events in the CD4+ lymphocyte gate is indicated. In the upper right quadrant the percentage of cytokine-producing cells is shown.