Figure 2

ELISA analysis of anti-B7-H4 mAb. - The lysate of 3T3-mB7-H4 cells, diluted to 10 μg/mL in 0.01 M coating solution concentrate, was coated onto high-binding polystyrene plates at 100 μL per well, and incubated at 4°C overnight. The lysate of 3T3 cells not transfected with B7-H4 was used as a control. The titer of 3E8 (at an original concentration of 1.5 mg/mL) was determined by ELISA and read at A₄₅₀.