Expression of B7-H4 in normal tissues and corresponding cancer samples from breast, uterus, and ovary. - Human normal and cancerous samples in tissue arrays were subjected to IHC staining using mAb 3E8. The reactions were visualized using reagents from a DAKO Envision System kit. Normal mouse IgM was used as an antibody control (data not shown). Samples were photographed and staining intensity (0 to 3, least intense to most intense) and the proportion of stained cells (0 to 4, no cells stained to more than 70% cells stained) were semi-quantitatively determined. A combined score of ≥6 was considered to indicate overexpression.