Figure 7
In vitro effects of PARP-1 inhibition on peripheral blood lymphocytes. Isolated spleen cells were incubated with 20 μg/ml oxLDL. The effects of addition of specific PARP-1 inhibitor INO-1001 on the expression of activation-associated cytokines were measured by qPCR after 8 h. cDNA was generated of incubated cells and molecule transcripts of various molecules were compared between INO-1001 treated und control animals. Copy numbers were adjusted for copies of the housekeeping gene β-actin. Representative data from one of three independent experiments with similar results.