Real-time PCR analyses of Barrett metaplasias (mucosa) and the adjacent Barrett carcinomas (tumor). Ct values were determined from duplicate reactions. ΔCt were obtained by subtracting the mean Ct value for GAPDH from the mean Ct value from E-cadherin. A lower ΔCt value indicates a higher gene expression. The horizontal bars indicate the mean values. The E-cadherin expression level was significantly different between the two groups (P = 0,02, Mann_Whitney U Test). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.