Construction of the bio-artificial liver support system. The system consists of three roller pumps, a heparin pump, an infusion heater, a plasmafilter, a plasma component separator, an oxygenation device, and a multi-layer radial-flow bioreactor containing galactosylated chitosan nanofiber scaffolds. The bioreactor and the oxygenation device is kept in an incubator with the internal temperature of 37°C. All devices are connected by sterile tubing. The dotted arrow indicates where the samples were drawn into the circuits.