Figure 4

Expressions of LC3 and beclin-1 in the BA walls in the control (n = 5, Lane 1), SAH (n = 5, Lane 2), SAH + vehicle (n = 5, Lane 3), SAH + low dose of CysC (n = 5, Lane 4), and SAH + high dose of CysC (n = 5, Lane 5) groups. Upper: Representative autoradiograph showing protein expression following SAH by western blot. We detected LC3 at 16 kDa, beclin-1 at 52 kDa, and the loading control glyceraldehyde-3-phosphate dehydrogenase at 36 kD. Bottom: Quantitative analysis of the western blot results for the levels of LC3 and beclin-1. The expression of autophagy-related proteins was low in the control group. The expression of autophagy proteins was significantly increased in the SAH and SAH + vehicle experimental groups compared with controls (P <0.05). The increased expression was further markedly upregulated by CysC treatment (P <0.01). *P <0.05 compared with control group; **P <0.01 compared with control group; ns P >0.05 compared with SAH + vehicle group; #P <0.05 compared with SAH + vehicle group; ##P <0.01 compared with SAH + vehicle group.