Figure 5

Electron micrographs of the cortex at 48 h following sham operation (A), SAH induction (B and C), vehicle treatment (D), and CysC treatment (E, F, G, and H). (A) In the control group, the glial cells were normal with integrated nuclear membrane. No swelling was found in endoplasmic reticulum and mitochondria. The electron density was normal in cytoplasm. (B) In the SAH group, the nuclear membrane was not integrated and the cytoplasm component entered the cell nucleus pushing the nuclear membrane. (C) In the SAH group, the nuclear membrane dissolved and shrank with nucleolar margination and chromatic agglutination in the glia cells. The staining was uneven with more endolysosome in the cytoplasm. (D) In the vehicle group, the endotheliocyte swelled in the capillary with apoptotic neurons and glial cells. (E) In the low dose CysC group, the nuclear membrane was more integrated than the SAH group with a little chromatic agglutination at the border of the nuclear membrane. In the cytoplasm, some of the mitochondria were swollen. (F) In the low dose CysC group, mild demyelination was found with the myelin sheath and mitochondria morphology was better than in the vehicle treated group. (G-H) In the high dose group, the myelin sheath was better than that in the SAH group surrounded with some stromal cells.