Table 1 Sequences of primers for real-time polymerase chain reaction (RT-PCR) amplification

Osteopontin (OPN)

Forward primer: 5′-TCACCAGTCTGATGAGTCTCAC-3′

Reverse primer: 5′-CAGGTCTGCGAAACTTCTTAGAT-3′

Bone sialoprotein (BSP)

Forward primer: 5′-GAATGGCCTGTGCTTTCTCAA-3′

Reverse primer: 5′-TCGGATGAGTCACTACTGCCC-3′

Integrin α5 subunit (α-V)

Forward primer: 5′-CCTCAGACGCTGCGTGGAGC-3′

Reverse primer: 5′-AGGGCTGAGCTTCGGAGCGA-3′

B cell lymphoma/leukemia-2 (Bcl2)

Sense primer: 5′-ATCCAGGATAACGGAGGC-3′

Antisense primer: 5′-CAGCCAGGAGAAATCAAAC-3′

Bcl-2 associated X protein (Bax)

Sense primer: 5′-GACCCGGTGCCTCAGGATGC-3′

Antisense primer: 5′-GTCTGTGTCCACGGCGGCAA-3′

Caspase 3

Sense primer: 5′-CATGGAAGCGAATCAATGGACT-3′

Antisense primer: 5′-CTGTACCAGACCGAGATGTCA-3′

β-Actin

Forward primer: 5′-GCAGAAGGAGATCACAGCCCT-3′

Reverse primer: 5′-GCTGATCCACATCTGCTGGAA-3′