miR-10b expression on A549 and H1299 cells. (A) Fluorescence photomicrographs of A549 cells infected by miR-10b at a multiplicity of transfection of 10. Images were taken 72 hours after infection. Scare bar = 100 μm. (B) miR-10b was subjected to real-time PCR analysis for miR-10b and U6 expression levels. Ectopic expression of miR-10b on A549 and H1299 cells was analyzed by qRT-PCR. Asterisks indicate significant difference when compared with control (P <0.05). miR-10b, microRNA-10b.