The expression level of ferroportin in different cell lines. A) Western blotting: 1, Normal human liver cell line L02; 2, FOCUS; 3, HepG2; 4, MHCC-97H; 5, SMMC-7721. βMactin was shown as the loading control. B) Statistical graph of the expression level of ferroportin in different cell lines. **The expression difference of ferroportin between normal human liver cell line and hepatocellular carcinoma (HCC) cell lines all had statistical significance, P <0.01. *The difference of ferroportin expression between MHCC-97H and other HCC cell lines had statistical significance, P <0.05 (for convenience, the single asterisk has only been shown between MHCC-97H and FOCUS; it is the same consequence for the comparison between MHCC-97H and HepG2/ SMMC-7721).