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Table 1 Comparison of apoptosis, necrosis and cell debris, and cell viability of cells preserved with different concentrations of dimethyl sulfoxide (DMSO)

From: Cryopreservation and replantation of amputated rat hind limbs

Groups

Apoptosis (%)

Necrosis and cell debris (%)

Cell viability (%)

 

Before

After

Before

After

Before

After

 

Cryopreservation

Cryopreservation

Cryopreservation

B0

7.7 ± 0.7

8.2 ± 5.9

2.8 ± 0.4

88.0 ± 6.2a

89.5 ± 3.1

3.8 ± 1.6a

B1

7.4 ± 0.8

7.4 ± 1.5

3.2 ± 0.9

3.8 ± 0.9

89.4 ± 3.7

88.8 ± 0.9

B2

8.1 ± 0.7

37.5 ± 2.1a

3.1 ± 0.7

4.3 ± 0.9

88.2 ± 3.5

58.3 ± 2.6a

  1. Data are presented as mean ± standard deviation.
  2. B0, 0% DMSO; B1, 10% DMSO; B2, 50% DMSO.
  3. Sample size = 7.
  4. aP < 0.05 compared to before cryopreservation.